ArticleThe Journal of cell biology1989
Regulation of desmosome assembly in epithelial cells: kinetics of synthesis, transport, and stabilization of desmoglein I, a major protein of the membrane core domain.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 128 citations in OpenAlex.
- Desmoglein-driven dynamic signaling in pemphigus vulgaris: a systematic review of pathogenic pathways.NPJ Regenerative medicine · 2025Article
- The desmosome comes into focus.The Journal of cell biology · 2024Review
- Desmosomes at a glance.Journal of cell science · 2024Review
- Differential Pathomechanisms of Desmoglein 1 Transmembrane Domain Mutations in Skin Disease.The Journal of investigative dermatology · 2022Article
- Glycan distribution and density in native skin's stratum corneum.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2018Article
- ER-to-Golgi blockade of nascent desmosomal cadherins in SERCA2-inhibited keratinocytes: Implications for Darier's disease.Traffic (Copenhagen, Denmark) · 2017Article
- Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation.Molecular biology of the cell · 2014Article
- Different roles of cadherins in the assembly and structural integrity of the desmosome complex.Journal of cell science · 2014Article
- Desmosome assembly and dynamics.Trends in cell biology · 2013Review
- Gingival crevicular fluid as a periodontal diagnostic indicator- II: Inflammatory mediators, host-response modifiers and chair side diagnostic aids.Journal of medicine and life · 2013Review
- Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes.The Journal of cell biology · 2011Article
- Desmoglein 2 mutant mice develop cardiac fibrosis and dilation.Basic research in cardiology · 2011Article
- Intercellular junction assembly, dynamics, and homeostasis.Cold Spring Harbor perspectives in biology · 2010Review
- Desmosomes in vivo.Dermatology research and practice · 2010Article
- Loss of desmocollin 3 in mice leads to epidermal blistering.Journal of cell science · 2008Article
- The desmosome and pemphigus.Histochemistry and cell biology · 2008Review
- Pemphigus vulgaris immunoglobulin G can recognize a 130 000 MW antigen other than desmoglein 3 on peripheral blood mononuclear cell surface.Immunology · 2007Article
- Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin.The Journal of cell biology · 2005Article
- Requirement of plakophilin 2 for heart morphogenesis and cardiac junction formation.The Journal of cell biology · 2004Article
- Assessment of splice variant-specific functions of desmocollin 1 in the skin.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Desmosomes are composed of two morphologically and biochemically distinct domains, a cytoplasmic plaque and membrane core. We have initiated a study of the synthesis and assembly of these domains in Madin-Darby canine kidney (MDCK) epithelial cells to understand the mechanisms involved in the formation of desmosomes. Previously, we reported the kinetics of assembly of two components of the cytoplasmic plaque domain, Desmoplakin I/II (Pasdar, M., and W. J. Nelson. 1988. J. Cell Biol. 106:677-685 and 106:687-699. We have now extended this analysis to include a major glycoprotein component of the membrane core domain, Desmoglein I (DGI; Mr = 150,000). Using metabolic labeling and inhibitors of glycoprotein processing and intracellular transport, we show that DGI biosynthesis is a sequential process with defined stages. In the absence of cell-cell contact, DGI enters a Triton X-100 soluble pool and is core glycosylated. The soluble DGI is then transported to the Golgi complex where it is first complex glycosylated and then titrated into an insoluble pool. The insoluble pool of DGI is subsequently transported to the plasma membrane and is degraded rapidly (t1/2 less than 4 h). Although this biosynthetic pathway occurs independently of cell-cell contact, induction of cell-cell contact results in dramatic increases in the efficiency and rate of titration of DGI from the soluble to the insoluble pool, and its transport to the plasma membrane where DGI becomes metabolically stable (t1/2 greater than 24 h). Taken together with our previous study of DPI/II, we conclude that newly synthesized components of the cytoplasmic plaque and membrane core domains are processed and assembled with different kinetics indicating that, at least initially, each domain is assembled separately in the cell. However, upon induction of cell-cell contact there is a rapid titration of both components into an insoluble and metabolically stable pool at the plasma membrane that is concurrent with desmosome assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.