ArticleJournal of experimental botany2014
Targeting of a polytopic membrane protein to the inner envelope membrane of chloroplasts in vivo involves multiple transmembrane segments.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The N-region sequence context impacts the chloroplast import efficiency of multi-TMD protein.Plant molecular biology · 2024Article
- Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns.Frontiers in plant science · 2021Review
- Induction of TOC and TIC genes during photomorphogenesis is mediated primarily by cryptochrome 1 in Arabidopsis.Scientific reports · 2020Article
- Installation of authentic BicA and SbtA proteins to the chloroplast envelope membrane is achieved by the proteolytic cleavage of chimeric proteins in Arabidopsis.Scientific reports · 2020Article
- A Chloroplast COR413 Protein FromFrontiers in plant science · 2020Article
- The Overexpression of a Transcription Factor GeneFrontiers in plant science · 2019Article
- Evolution of protein transport to the chloroplast envelope membranes.Photosynthesis research · 2018Review
- PsCor413pm2, a Plasma Membrane-Localized, Cold-Regulated Protein fromInternational journal of molecular sciences · 2018Article
- Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes.Journal of experimental botany · 2018Article
- Evolution and Design Principles of the Diverse Chloroplast Transit Peptides.Molecules and cells · 2018Review
- Investigating Localization of Chimeric Transporter Proteins within Chloroplasts ofBio-protocol · 2018Article
- Prolines in Transit Peptides Are Crucial for Efficient Preprotein Translocation into Chloroplasts.Plant physiology · 2018Article
- Setting sub-organellar sights: accurate targeting of multi-transmembrane-domain proteins to specific chloroplast membranes.Journal of experimental botany · 2017Article
- Sorting of SEC translocase SCY components to different membranes in chloroplasts.Journal of experimental botany · 2017Article
- Specific and Efficient Targeting of Cyanobacterial Bicarbonate Transporters to the Inner Envelope Membrane of Chloroplasts in Arabidopsis.Frontiers in plant science · 2016Article
- The C-terminus of Bienertia sinuspersici Toc159 contains essential elements for its targeting and anchorage to the chloroplast outer membrane.Frontiers in plant science · 2014Article
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Authors and funding
8 authors.
Funding
Abstract
The inner envelope membrane (IEM) of the chloroplast plays crucial roles in forming an osmotic barrier and controlling metabolite exchange between the organelle and the cytosol. The IEM therefore harbours a number of membrane proteins and requires the import and integration of these nuclear-encoded proteins for its biogenesis. Recent studies have demonstrated that the transmembrane segment of single-spanning IEM proteins plays key roles in determining their IEM localization. However, few studies have focused on the molecular mechanisms by which polytopic membrane proteins are targeted to the IEM. In this study, we investigated the targeting mechanism of polytopic IEM proteins using the protein Cor413im1 as a model substrate. Cor413im1 does not utilize a soluble intermediate for its targeting to the IEM. Furthermore, we show that the putative fifth transmembrane segment of Cor413im1 is necessary for its targeting to the IEM. The C-terminal portion containing this transmembrane segment is also able to deliver Cor413im1 protein to the IEM. However, the fifth transmembrane segment of Cor413im1 itself is insufficient to target a fusion protein to the IEM. These data suggest that the targeting of polytopic membrane proteins to the chloroplast IEM in vivo involves multiple transmembrane segments and that chloroplasts have evolved a unique mechanism for the integration of polytopic proteins to the IEM.
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