Evidence map›Paper›PMID 25012948›Full record

ReviewJournal of clinical pathology2014

Guidance for laboratories performing molecular pathology for cancer patients.

Ian A Cree, Zandra Deans, Marjolijn J L Ligtenberg, Nicola Normanno, Anders Edsjö, Etienne Rouleau, Francesc Solé, Erik Thunnissen, Wim Timens, Ed Schuuring and 7 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of clinical pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
99citing papers in PubMed, 5 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

99 citing papers in PubMed, 5 syntheses or guidelines pooled it.

  1. Guideline
  2. Guideline
  3. Guideline
  4. Guideline
  5. Pooled it
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Toward Optimizing the Impact of Digital Pathology and Augmented Intelligence on Issues of Diagnosis, Grading, Staging, and Classification.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2025
    Review
  15. Custom RJournal of pathology informatics · 2025
    Article
  16. Article
  17. Review
  18. Review
  19. Article
  20. Review

39 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Ian A CreeWarwick Medical School, University Hospital Coventry and Warwickshire, Coventry, UK Institute of Ophthalmology, University College London, London, UK.ORCID http://orcid.org/0000-0001-5007-9456
Zandra DeansUK NEQAS for Molecular Genetics, Department of Laboratory Medicine, Royal Infirmary of Edinburgh, Edinburgh, UK.
Marjolijn J L LigtenbergDepartment of Pathology 824, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Nicola NormannoCell Biology and Biotherapy Unit, INT-Fondazione Pascale, Naples, Italy.
Anders EdsjöClinical Molecular Pathology Unit, Clinical Pathology and Genetics, Sahlgrenska University Hospital and Sahlgrenska Cancer Center, the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Etienne RouleauService de Génétique, Unités de Génétique constitutionnelle et somatique, Paris, France.
Francesc SoléInstitut de Recerca contra la Leucèmia Josep Carreras (IJC), Barcelona, Spain.
Erik ThunnissenDepartment of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
Wim TimensDepartment of Pathology and Medical Biology, University Medical Center Groningen, Groningen, The Netherlands.
Ed SchuuringDepartment of Pathology and Medical Biology, University Medical Center Groningen, Groningen, The Netherlands.
Elisabeth DequekerDepartment of Public Health and Primary Care, Biomedical Quality Assurance Research Unit, KU Leuven-University of Leuven, Leuven, Belgium.
Samuel MurrayBiomarker Solutions Ltd, London, UK.
Manfred DietelInstitute of Pathology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Patricia GroenenDepartment of Pathology 824, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
J Han Van KriekenDepartment of Pathology 824, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
European Society of Pathology Task Force on Quality Assurance in Molecular Pathology
Royal College of Pathologists

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular testing is becoming an important part of the diagnosis of any patient with cancer. The challenge to laboratories is to meet this need, using reliable methods and processes to ensure that patients receive a timely and accurate report on which their treatment will be based. The aim of this paper is to provide minimum requirements for the management of molecular pathology laboratories. This general guidance should be augmented by the specific guidance available for different tumour types and tests. Preanalytical considerations are important, and careful consideration of the way in which specimens are obtained and reach the laboratory is necessary. Sample receipt and handling follow standard operating procedures, but some alterations may be necessary if molecular testing is to be performed, for instance to control tissue fixation. DNA and RNA extraction can be standardised and should be checked for quality and quantity of output on a regular basis. The choice of analytical method(s) depends on clinical requirements, desired turnaround time, and expertise available. Internal quality control, regular internal audit of the whole testing process, laboratory accreditation, and continual participation in external quality assessment schemes are prerequisites for delivery of a reliable service. A molecular pathology report should accurately convey the information the clinician needs to treat the patient with sufficient information to allow for correct interpretation of the result. Molecular pathology is developing rapidly, and further detailed evidence-based recommendations are required for many of the topics covered here.

Indexed as

BenchmarkingBiomarkers, TumorGenetic TestingGuideline AdherenceHumansLaboratoriesMedical OncologyMedical RecordsNeoplasmsPathology, MolecularPractice Guidelines as TopicPredictive Value of TestsPrognosisSpecimen HandlingBiomarkers, TumorLaboratory TestsMolecular OncologyMolecular PathologyNeoplasmsQuality Control

Identifiers

PMID25012948
PMCPMC4215286

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.