ArticleThe ISME journal2014
Widespread expression of conserved small RNAs in small symbiont genomes.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- A Meta-Analysis ofG3 (Bethesda, Md.) · 2020Pooled it
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- Host-specific co-evolution likely driven by diet in Buchnera aphidicola.BMC genomics · 2024Article
- Coordination of host and endosymbiont gene expression governs endosymbiont growth and elimination in the cereal weevil Sitophilus spp.Microbiome · 2023Article
- Co-option of a conserved host glutamine transporter facilitates aphid/Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Absence of microbiome triggers extensive changes in the transcriptional profile of Hermetia illucens during larval ontology.Scientific reports · 2023Article
- Epigenetic regulations as drivers of insecticide resistance and resilience to climate change in arthropod pests.Frontiers in genetics · 2022Review
- The Tsetse Metabolic Gambit: Living on Blood by Relying on Symbionts Demands Synchronization.Frontiers in microbiology · 2022Review
- Potato leafroll virus reduces Buchnera aphidocola titer and alters vector transcriptome responses.Scientific reports · 2021Article
- Natural Selection Shapes Maintenance of Orthologous sRNAs in Divergent Host-Restricted Bacterial Genomes.Molecular biology and evolution · 2021Article
- Pantothenate mediates the coordination of whitefly and symbiont fitness.The ISME journal · 2021Article
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- Sensing, Signaling, and Secretion: A Review and Analysis of Systems for Regulating Host Interaction inGenes · 2020Review
- Coordination of host and symbiont gene expression reveals a metabolic tug-of-war between aphids andProceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome architecture of a microbe markedly changes when it transitions from a free-living lifestyle to an obligate symbiotic association within eukaryotic cells. These symbiont genomes experience numerous rearrangements and massive gene loss, which is expected to radically alter gene regulatory networks compared with those of free-living relatives. As such, it remains unclear whether and how these small symbiont genomes regulate gene expression. Here, using a label-free mass-spec quantification approach we found that differential protein regulation occurs in Buchnera, a model symbiont with a reduced genome, when it transitions between two distinct life stages. However, differential mRNA expression could not be detected between Buchnera life stages, despite the presence of a small number of putative transcriptional regulators. Instead a comparative analysis of small RNA expression profiles among five divergent Buchnera lineages, spanning a variety of Buchnera life stages, reveals 140 novel intergenic and antisense small RNAs and 517 untranslated regions that were significantly expressed, some of which have been conserved for ∼65 million years. In addition, the majority of these small RNAs exhibit both sequence covariation and thermodynamic stability, indicators of a potential structural RNA role. Together, these data suggest that gene regulation at the post-transcriptional level may be important in Buchnera. This is the first study to empirically identify Buchnera small RNAs, and we propose that these novel small RNAs may facilitate post-transcriptional regulation through translational inhibition/activation, and/or transcript stability. Ultimately, post-transcriptional regulation may shape metabolic complementation between Buchnera and its aphid host, thus impacting the animal's ecology and evolution.
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