ArticleThe ISME journal2015
New insights into iron acquisition by cyanobacteria: an essential role for ExbB-ExbD complex in inorganic iron uptake.
Article in The ISME journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Cyanobacteria's power trio: auxin, siderophores, and nitrogen fixation to foster thriving agriculture.World journal of microbiology & biotechnology · 2024Pooled it
- Iron biofortification as a promising strategy to improve productivity and nutritional value of Arthrospira platensis (spirulina).Scientific reports · 2026Article
- Iron binding before iron limitation: siderophore synthesis arose well before iron became a limiting element.ISME communications · 2026Article
- Molecular mechanism of a coastal cyanobacteriumMarine life science & technology · 2024Article
- A global transcriptional activator involved in the iron homeostasis in cyanobacteria.Science advances · 2024Article
- Hypothetical chloroplast reading frame 51 encodes a photosystem I assembly factor in cyanobacteria.The Plant cell · 2024Article
- A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria.Nature communications · 2024Article
- Interactions between the nitrogen-fixing cyanobacteriumISME communications · 2024Article
- Proteomics analysis reveals differential acclimation of coastal and oceanicFrontiers in microbiology · 2024Article
- Microbial iron acquisition is influenced by spatial and temporal conditions in a glacial influenced river and estuary system.Environmental microbiology · 2023Article
- Diversity and Evolution of Iron Uptake Pathways in Marine Cyanobacteria from the Perspective of the Coastal StrainApplied and environmental microbiology · 2023Article
- A Four-Step Platform to Optimize Growth Conditions for High-Yield Production of Siderophores in Cyanobacteria.Metabolites · 2023Article
- Elucidation of the coping strategy in an OMP homozygous knockout mutant of Synechocystis 6803 defective in iron uptake.Archives of microbiology · 2022Article
- Genome-wide identification and characterization of Fur-binding sites in the cyanobacteria Synechocystis sp. PCC 6803 and PCC 6714.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Iron-Fueled Life in the Continental Subsurface: Deep Mine Microbial Observatory, South Dakota, USA.Applied and environmental microbiology · 2021Article
- Article
- New insights into the function of the proteins IsiC and IsiD from Synechocystis sp. PCC 6803 under iron limitation.Applied microbiology and biotechnology · 2021Article
- The Thermosynechococcus Genus: Wide Environmental Distribution, but a Highly Conserved Genomic Core.Microbes and environments · 2021Article
- From the Ocean to the Lab-Assessing Iron Limitation in Cyanobacteria: An Interface Paper.Microorganisms · 2020Review
- Siderophore piracy enhancesMicrobiology (Reading, England) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are globally important primary producers that have an exceptionally large iron requirement for photosynthesis. In many aquatic ecosystems, the levels of dissolved iron are so low and some of the chemical species so unreactive that growth of cyanobacteria is impaired. Pathways of iron uptake through cyanobacterial membranes are now being elucidated, but the molecular details are still largely unknown. Here we report that the non-siderophore-producing cyanobacterium Synechocystis sp. PCC 6803 contains three exbB-exbD gene clusters that are obligatorily required for growth and are involved in iron acquisition. The three exbB-exbDs are redundant, but single and double mutants have reduced rates of iron uptake compared with wild-type cells, and the triple mutant appeared to be lethal. Short-term measurements in chemically well-defined medium show that iron uptake by Synechocystis depends on inorganic iron (Fe') concentration and ExbB-ExbD complexes are essentially required for the Fe' transport process. Although transport of iron bound to a model siderophore, ferrioxamine B, is also reduced in the exbB-exbD mutants, the rate of uptake at similar total [Fe] is about 800-fold slower than Fe', suggesting that hydroxamate siderophore iron uptake may be less ecologically relevant than free iron. These results provide the first evidence that ExbB-ExbD is involved in inorganic iron uptake and is an essential part of the iron acquisition pathway in cyanobacteria. The involvement of an ExbB-ExbD system for inorganic iron uptake may allow cyanobacteria to more tightly maintain iron homeostasis, particularly in variable environments where iron concentrations range from limiting to sufficient.
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