ArticleAnalytical and bioanalytical chemistry2015
On chip preconcentration and fluorescence labeling of model proteins by use of monolithic columns: device fabrication, optimization, and automation.
Article in Analytical and bioanalytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Development of a Thiol-ene Microfluidic Chip for Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS).Analytical chemistry · 2025Article
- 3D Printed Microfluidic Devices for Solid-Phase Extraction and On-Chip Fluorescent Labeling of Preterm Birth Risk Biomarkers.Analytical chemistry · 2020Article
- Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis.Analytical and bioanalytical chemistry · 2020Review
- Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis.Analytical and bioanalytical chemistry · 2018Article
- Electrokinetically operated microfluidic devices for integrated immunoaffinity monolith extraction and electrophoretic separation of preterm birth biomarkers.The Analyst · 2017Article
- Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review.Analytica chimica acta · 2017Review
- Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers.Electrophoresis · 2017Article
- Rapid labeling of amino acid neurotransmitters with a fluorescent thiol in the presence of o-phthalaldehyde.Electrophoresis · 2017Article
- On-Chip Fluorescent Labeling using Reversed-phase Monoliths and Microchip Electrophoretic Separations of Selected Preterm Birth Biomarkers.Analytical methods : advancing methods and applications · 2016Article
- Methacrylate Polymer Monoliths for Separation Applications.Materials (Basel, Switzerland) · 2016Review
- Advances in monoliths and related porous materials for microfluidics.Biomicrofluidics · 2016Review
- Development of an integrated microfluidic solid-phase extraction and electrophoresis device.The Analyst · 2016Article
- Pressure-actuated microfluidic devices for electrophoretic separation of pre-term birth biomarkers.Analytical and bioanalytical chemistry · 2016Article
- Advances in and prospects of microchip liquid chromatography.Trends in analytical chemistry : TRACArticle
Corrections and comments
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4 authors.
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Abstract
Microfluidic systems with monolithic columns have been developed for preconcentration and on-chip labeling of model proteins. Monoliths were prepared in microchannels by photopolymerization, and their properties were optimized by varying the composition and concentration of the monomers to improve flow and extraction. On-chip labeling of proteins was achieved by driving solutions through the monolith by use of voltage then incubating fluorescent dye with protein retained on the monolith. Subsequently, the labeled proteins were eluted, by applying voltages to reservoirs on the microdevice, and then detected, by monitoring laser-induced fluorescence. Monoliths prepared from octyl methacrylate combine the best protein retention with the possibility of separate elution of unattached fluorescent label with 50% acetonitrile. Finally, automated on-chip extraction and fluorescence labeling of a model protein were successfully demonstrated. This method involves facile sample pretreatment, and therefore has potential for production of integrated bioanalysis microchips.
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