ArticleAnalytical and bioanalytical chemistry2014
Real-time monitoring of immobilized single yeast cells through multifrequency electrical impedance spectroscopy.
Article in Analytical and bioanalytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- AI-Assisted Impedance Biosensing of Yeast Cell Concentration.Biosensors · 2025Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- Real-Time Monitoring of Dissection Events of Single Budding Yeast in a Microfluidic Cell-Culturing Device Integrated With Electrical Impedance Biosensor.Frontiers in bioengineering and biotechnology · 2021Article
- A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry.Analytical and bioanalytical chemistry · 2020Article
- Article
- Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems.ACS chemical biology · 2018Review
- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo.Methods in molecular biology (Clifton, N.J.) · 2018Review
- Review
- Recent Advances in the Analysis of Single Cells.Analytical chemistry · 2017Review
- A Versatile Bonding Method for PDMS and SU-8 and Its Application towards a Multifunctional Microfluidic Device.Micromachines · 2016Article
- Dynamic monitoring of single cell lysis in an impedance-based microfluidic device.Biomedical microdevices · 2016Article
- Single cell studies of mouse embryonic stem cell (mESC) differentiation by electrical impedance measurements in a microfluidic device.Biosensors & bioelectronics · 2016Article
- Time-lapse electrical impedance spectroscopy for monitoring the cell cycle of single immobilized S. pombe cells.Scientific reports · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
We present a microfluidic device, which enables single cells to be reliably trapped and cultivated while simultaneously being monitored by means of multifrequency electrical impedance spectroscopy (EIS) in the frequency range of 10 kHz-10 MHz. Polystyrene beads were employed to characterize the EIS performance inside the microfluidic device. The results demonstrate that EIS yields a low coefficient of variation in measuring the diameters of captured beads (~0.13%). Budding yeast, Saccharomyces cerevisiae, was afterwards used as model organism. Single yeast cells were immobilized and measured by means of EIS. The bud growth was monitored through EIS at a temporal resolution of 1 min. The size increment of the bud, which is difficult to determine optically within a short time period, can be clearly detected through EIS signals. The impedance measurements also reflect the changes in position or motion of single yeast cells in the trap. By analyzing the multifrequency EIS data, cell motion could be qualitatively discerned from bud growth. The results demonstrate that single-cell EIS can be used to monitor cell growth, while also detecting potential cell motion in real-time and label-free approach, and that EIS constitutes a sensitive tool for dynamic single-cell analysis.
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Registered trials
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