ArticleNature protocols2014
Quantitative analysis of triple-mutant genetic interactions.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- CRISPRi-TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria.Nature microbiology · 2024Article
- Gradients in gene essentiality reshape antibacterial research.FEMS microbiology reviews · 2022Review
- τ-SGA: synthetic genetic array analysis for systematically screening and quantifying trigenic interactions in yeast.Nature protocols · 2021Article
- Mutation Edgotype Drives Fitness Effect in Human.Frontiers in bioinformatics · 2021Article
- Genetic interaction mapping informs integrative structure determination of protein complexes.Science (New York, N.Y.) · 2020Article
- Article
- High-Throughput Quantitative Genetic Interaction Mapping in the Fission YeastCold Spring Harbor protocols · 2018Article
- Systematic Gene-to-Phenotype Arrays: A High-Throughput Technique for Molecular Phenotyping.Molecular cell · 2018Article
- Suppressive drug combinations and their potential to combat antibiotic resistance.The Journal of antibiotics · 2017Review
- Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.Scientific reports · 2017Article
- Hypermutation signature reveals a slippage and realignment model of translesion synthesis by Rev3 polymerase in cisplatin-treated yeast.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe.Open biology · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The quantitative analysis of genetic interactions between pairs of gene mutations has proven to be effective for characterizing cellular functions, but it can miss important interactions for functionally redundant genes. To address this limitation, we have developed an approach termed triple-mutant analysis (TMA). The procedure relies on a query strain that contains two deletions in a pair of redundant or otherwise related genes, which is crossed against a panel of candidate deletion strains to isolate triple mutants and measure their growth. A central feature of TMA is to interrogate mutants that are synthetically sick when two other genes are deleted but interact minimally with either single deletion. This approach has been valuable for discovering genes that restore critical functions when the principal actors are deleted. TMA has also uncovered double-mutant combinations that produce severe defects because a third protein becomes deregulated and acts in a deleterious fashion, and it has revealed functional differences between proteins presumed to act together. The protocol is optimized for Singer ROTOR pinning robots, takes 3 weeks to complete and measures interactions for up to 30 double mutants against a library of 1,536 single mutants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.