ArticleAnalytical chemistry2014
Reagentless, ratiometric electrochemical DNA sensors with improved robustness and reproducibility.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- A portable and cost-effective system for electronic nucleic acid mass measurement.Scientific reports · 2025Article
- Dual-Frequency, Ratiometric Approaches to EAB Sensor Interrogation Support the Calibration-Free Measurement of Specific Molecules In Vivo.ACS sensors · 2024Article
- A Dual-Signaling Electrochemical Aptasensor Based on an In-Plane Gold Nanoparticles-Black Phosphorus Heterostructure for the Sensitive Detection of Patulin.Foods (Basel, Switzerland) · 2023Article
- Theoretical Design of a Janus-Nanoparticle-Based Sandwich Assay for Nucleic Acids.International journal of molecular sciences · 2022Article
- A label-free and modification-free ratiometric electrochemical strategy for enhanced natural enzyme detection using a bare electrode and nanozymes system.Analytical and bioanalytical chemistry · 2022Article
- Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors.Molecules (Basel, Switzerland) · 2021Review
- A Simple Colorimetric Assay for Sensitive CuFrontiers in chemistry · 2021Article
- Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices.Sensors (Basel, Switzerland) · 2020Review
- An electrochemical aptasensor for amyloid-β oligomer based on double-stranded DNA as "conductive spring".Mikrochimica acta · 2020Article
- High frequency, calibration-free molecular measurementsChemical science · 2019Article
- Nucleic acid-based ratiometric electrochemiluminescent, electrochemical and photoelectrochemical biosensors: a review.Mikrochimica acta · 2019Review
- Aptamer based ratiometric electrochemical sensing of 17β-estradiol using an electrode modified with gold nanoparticles, thionine, and multiwalled carbon nanotubes.Mikrochimica acta · 2019Article
- Survey of Redox-Active Moieties for Application in Multiplexed Electrochemical Biosensors.Analytical chemistry · 2016Article
- Ratiometric ultrasensitive electrochemical immunosensor based on redox substrate and immunoprobe.Scientific reports · 2016Article
- Facile construction of a highly sensitive DNA biosensor by in-situ assembly of electro-active tags on hairpin-structured probe fragment.Scientific reports · 2016Article
- Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.Scientific reports · 2015Article
- Electrochemical sensors and biosensors based on nanomaterials and nanostructures.Analytical chemistry · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
To make the electrochemical DNA sensors (E-sensor) more robust and reproducible, we have now for the first time adapted the techniques of ratiometric analyses to the field of E-sensors. We did this via the simple expedient way of simultaneously using two redox probes: Methylene blue as the reporter of the conformational change, and ferrocene as an internal control. During the conformational transduction, only the distance between the signal probe and the electrode surface undergoes an appreciable change, while the distance between the control probe and the electrode remains relatively constant. This special design has allowed very reliable target recognition, as illustrated in this report using a human T-lymphotropic virus type I gene fragment. The standard deviation between measurements obtained using different electrodes was an order of magnitude less than that obtained using a classic E-sensor, which we prepared as a control. A limit of detection of 25.1 pM was obtained with our new system, with a single mismatch discrimination factor of 2.33 likewise being observed. Additionally, this concept had general applicability, and preliminary data of a "Signal-On" ratiometric E-sensor are also provided. Taken in concert, these results serve to validate the utility of what we believe will emerge as an easily generalized approach to oligonucleotide recognition and sensing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.