ArticlePlant & cell physiology2014
Microtubule initiation from the nuclear surface controls cortical microtubule growth polarity and orientation in Arabidopsis thaliana.
Article in Plant & cell physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Microtubules in Arabidopsis pollen tubes are oriented away from the tube apex and are actin-independent at the cortex.bioRxiv : the preprint server for biology · 2026Article
- Transverse cortical microtubule arrays form persistent unipolar domains in hypocotyl cells ofMolecular biology of the cell · 2025Article
- A transient radial cortical microtubule array primes cell division inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Kinesin-4 optimizes microtubule orientations for responsive tip growth guidance in moss.The Journal of cell biology · 2023Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Microtubule associated protein WAVE DAMPENED2-LIKE (WDL) controls microtubule bundling and the stability of the site of tip-growth in Marchantia polymorpha rhizoids.PLoS genetics · 2021Article
- GR24, A Synthetic Strigolactone Analog, and Light Affect the Organization of Cortical Microtubules in Arabidopsis Hypocotyl Cells.Frontiers in plant science · 2021Article
- Article
- Elongation and shape changes in organisms with cell walls: A dialogue between experiments and models.Cell surface (Amsterdam, Netherlands) · 2018Review
- The self-organization of plant microtubules inside the cell volume yields their cortical localization, stable alignment, and sensitivity to external cues.PLoS computational biology · 2018Article
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- Microtubule Array Patterns Have a Common Underlying Architecture in Hypocotyl Cells.Plant physiology · 2018Article
- Article
- Regulation of microtubule nucleation mediated by γ-tubulin complexes.Protoplasma · 2017Review
- Distinct molecular cues ensure a robust microtubule-dependent nuclear positioning in the Drosophila oocyte.Nature communications · 2017Article
- Microtubule encounter-based catastrophe in Arabidopsis cortical microtubule arrays.BMC plant biology · 2016Article
- Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Microtubules in plants.The arabidopsis book · 2015Article
- The novel and taxonomically restricted Ah24 gene from grain amaranth (Amaranthus hypochondriacus) has a dual role in development and defense.Frontiers in plant science · 2015Article
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2 authors.
Funding
Abstract
The nuclear envelope in plant cells has long been known to be a microtubule organizing center (MTOC), but its influence on microtubule organization in the cell cortex has been unclear. Here we show that nuclear MTOC activity favors the formation of longitudinal cortical microtubule (CMT) arrays. We used green fluorescent protein (GFP)-tagged gamma tubulin-complex protein 2 (GCP2) to identify nuclear MTOC activity and GFP-tagged End-Binding Protein 1b (EB1b) to track microtubule growth directions. We found that microtubules initiate from nuclei and enter the cortex in two directions along the long axis of the cell, creating bipolar longitudinal CMT arrays. Such arrays were observed in all cell types showing nuclear MTOC activity, including root hairs, recently divided cells in root tips, and the leaf epidermis. In order to confirm the causal nature of nuclei in bipolar array formation, we displaced nuclei by centrifugation, which generated a corresponding shift in the bipolarity split point. We also found that bipolar CMT arrays were associated with bidirectional trafficking of vesicular components to cell ends. Together, these findings reveal a conserved function of plant nuclear MTOCs and centrosomes/spindle pole bodies in animals and fungi, wherein all structures serve to establish polarities in microtubule growth.
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