ArticleOsteoarthritis and cartilage2014
Metabolic enrichment of omega-3 polyunsaturated fatty acids does not reduce the onset of idiopathic knee osteoarthritis in mice.
Article in Osteoarthritis and cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Sex Specific Determinants in Osteoarthritis: A Systematic Review of Preclinical Studies.International journal of molecular sciences · 2020Pooled it
- Subchondral H-type blood vessel formation aggravates articular cartilage degeneration through LEP-LEPR axis.Frontiers in medicine · 2026Article
- Polyunsaturated fatty acids may not be helpful for people with osteoarthritis: a two-sample Mendelian randomization analysis.Scientific reports · 2025Article
- Gene therapy forProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeting the Inflammatory Hallmarks of Obesity-Associated Osteoarthritis: Towards Nutraceutical-Oriented Preventive and Complementary Therapeutic Strategies Based on n-3 Polyunsaturated Fatty Acids.International journal of molecular sciences · 2023Review
- Fundamentals of OA. An initiative of Osteoarthritis and Cartilage. Obesity and metabolic factors in OA.Osteoarthritis and cartilage · 2022Review
- Bioinformatics-Led Discovery of Osteoarthritis Biomarkers and Inflammatory Infiltrates.Frontiers in immunology · 2022Article
- Fatty Acids and Oxylipins in Osteoarthritis and Rheumatoid Arthritis-a Complex Field with Significant Potential for Future Treatments.Current rheumatology reports · 2021Review
- Transgenic conversion of ω-6 to ω-3 polyunsaturated fatty acids via fat-1 reduces the severity of post-traumatic osteoarthritis.Arthritis research & therapy · 2020Article
- Does a high-fat diet affect the development and progression of osteoarthritis in mice?: A systematic review.Bone & joint research · 2019Article
- Article
- Effects of dietary fatty acid content on humeral cartilage and bone structure in a mouse model of diet-induced obesity.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Physiologic and pathologic effects of dietary free fatty acids on cells of the joint.Annals of the New York Academy of Sciences · 2019Review
- Modern-day environmental factors in the pathogenesis of osteoarthritis.Nature reviews. Rheumatology · 2018Review
- Independent effects of dietary fat and sucrose content on chondrocyte metabolism and osteoarthritis pathology in mice.Disease models & mechanisms · 2018Article
- Profibrotic Infrapatellar Fat Pad Remodeling Without M1 Macrophage Polarization Precedes Knee Osteoarthritis in Mice With Diet-Induced Obesity.Arthritis & rheumatology (Hoboken, N.J.) · 2017Article
- Review: Metabolic Regulation of Inflammation in Osteoarthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2017Review
- Aging Promotes Sirtuin 3-Dependent Cartilage Superoxide Dismutase 2 Acetylation and Osteoarthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2016Article
- Osteoarthritis year in review 2015: biology.Osteoarthritis and cartilage · 2016Review
- Sunflower Oil but Not Fish Oil Resembles Positive Effects of Virgin Olive Oil on Aged Pancreas after Life-Long Coenzyme Q Addition.International journal of molecular sciences · 2015Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveWe evaluated the effect of a reduction in the systemic ratio of n-6:n-3 polyunsaturated fatty acids (PUFAs) on changes in inflammation, glucose metabolism, and the idiopathic development of knee osteoarthritis (OA) in mice. We hypothesized that a lower ratio of n-6:n-3 PUFAs would protect against OA markers in cartilage and synovium, but not bone.
designMale and female fat-1 transgenic mice (Fat-1), which convert dietary n-6 to n-3 PUFAs endogenously, and their wild-type (WT) littermates were fed an n-6 PUFA enriched diet for 9-14 months. The effect of gender and genotype on serum PUFAs, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and glucose tolerance was tested by 2-factor analysis of variance (ANOVA). Cortical and trabecular subchondral bone changes were documented by micro-focal computed tomography (CT), and knee OA was assessed by semi-quantitative histomorphometry grading.
resultsThe n-6:n-3 ratio was reduced 12-fold and 7-fold in male and female Fat-1 mice, respectively, compared to WT littermates. IL-6 and TNF-α levels were reduced modestly in Fat-1 mice. However, these systemic changes did not reduce osteophyte development, synovial hyperplasia, or cartilage degeneration. Also the fat-1 transgene did not alter subchondral cortical or trabecular bone morphology or bone mineral density.
conclusionsReducing the systemic n-6:n-3 ratio does not slow idiopathic changes in cartilage, synovium, or bone associated with early-stage knee OA in mice. The anti-inflammatory and anti-catabolic effects of n-3 PUFAs previously reported for cartilage may be more evident at later stages of disease or in post-traumatic and other inflammatory models of OA.
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Registered trials
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