Evidence map›Paper›PMID 25008204›Full record

ArticleOsteoarthritis and cartilage2014

Osteochondral defect repair using bilayered hydrogels encapsulating both chondrogenically and osteogenically pre-differentiated mesenchymal stem cells in a rabbit model.

J Lam, S Lu, E J Lee, J E Trachtenberg, V V Meretoja, R L Dahlin, J J J P van den Beucken, Y Tabata, M E Wong, J A Jansen and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Osteoarthritis and cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 56 citations in OpenAlex.

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  10. Evaluation of an Autologous Bone Mesenchymal Stem Cell-Derived Extracellular Matrix Scaffold in a Rabbit and Minipig Model of Cartilage Repair.Medical science monitor : international medical journal of experimental and clinical research · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

J LamDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA.
S LuDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA.
E J LeeDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA.
J E TrachtenbergDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA.
V V MeretojaDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA.
R L DahlinDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA.
J J J P van den BeuckenDepartment of Biomaterials, Radboud umc, Nijmegen, The Netherlands.
Y TabataDepartment of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.
M E WongDepartment of Surgery, Division of Oral and Maxillofacial Surgery, The University of Texas School of Dentistry, Houston, TX, USA.
J A JansenDepartment of Biomaterials, Radboud umc, Nijmegen, The Netherlands.
A G MikosDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA. Electronic address: mikos@rice.edu.
F K KasperDepartment of Bioengineering, Rice University, P.O. Box 1892, MS-142, Houston, TX 77251-1892, USA. Electronic address: kasper@rice.edu.
Rice University · USRadboud University Nijmegen · NLKyoto University · JPThe University of Texas at Austin · US

Funding

Injectable Cellular Composites for Cartilage EngineeringR01AR048756 · NIAMS · RICE UNIVERSITY · PI KASPER, FRED KURTIS, MIKOS, ANTONIOS G. · 2003 to 2014
$3.3M
NIAMS NIH HHS R01 AR048756
6 · The paper itself

Abstract

objectiveTo investigate the ability of cell-laden bilayered hydrogels encapsulating chondrogenically and osteogenically (OS) pre-differentiated mesenchymal stem cells (MSCs) to effect osteochondral defect repair in a rabbit model. By varying the period of chondrogenic pre-differentiation from 7 (CG7) to 14 days (CG14), the effect of chondrogenic differentiation stage on osteochondral tissue repair was also investigated.

methodsRabbit MSCs were subjected to either chondrogenic or osteogenic pre-differentiation, encapsulated within respective chondral/subchondral layers of a bilayered hydrogel construct, and then implanted into femoral condyle osteochondral defects. Rabbits were randomized into one of four groups (MSC/MSC, MSC/OS, CG7/OS, and CG14/OS; chondral/subchondral) and received two similar constructs bilaterally. Defects were evaluated after 12 weeks.

resultsAll groups exhibited similar overall neo-tissue filling. The delivery of OS cells when compared to undifferentiated MSCs in the subchondral construct layer resulted in improvements in neo-cartilage thickness and regularity. However, the addition of CG cells in the chondral layer, with OS cells in the subchondral layer, did not augment tissue repair as influenced by the latter when compared to the control. Instead, CG7/OS implants resulted in more irregular neo-tissue surfaces when compared to MSC/OS implants. Notably, the delivery of CG7 cells, when compared to CG14 cells, with OS cells stimulated morphologically superior cartilage repair. However, neither osteogenic nor chondrogenic pre-differentiation affected detectable changes in subchondral tissue repair.

conclusionsCartilage regeneration in osteochondral defects can be enhanced by MSCs that are chondrogenically and osteogenically pre-differentiated prior to implantation. Longer chondrogenic pre-differentiation periods, however, lead to diminished cartilage repair.

Indexed as

Absorbable ImplantsAnimalsCartilage, ArticularCell DifferentiationCells, CulturedChondrogenesisDisease Models, AnimalFemurHydrogelsMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationOsteogenesisRabbitsTime FactorsHydrogelsChondrogenicHydrogelMesenchymal stem cellOsteochondral repairOsteogenicPre-differentiation

Identifiers

PMID25008204
PMCPMC4150851
OpenAlexW2144884258

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.