ArticleJournal of the Royal Society, Interface2014
Droplet interface bilayer reconstitution and activity measurement of the mechanosensitive channel of large conductance from Escherichia coli.
Article in Journal of the Royal Society, Interface, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A simple method to make, trap and deform a vesicle in a gel.Scientific reports · 2023Article
- Challenges and opportunities in achieving the full potential of droplet interface bilayers.Nature chemistry · 2022Review
- Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies.Soft matter · 2022Article
- Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry.Chemical science · 2021Article
- Membrane protein mediated bilayer communication in networks of droplet interface bilayers.Communications chemistry · 2020Article
- Probing membrane protein properties using droplet interface bilayers.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Measuring bilayer surface energy and curvature in asymmetric droplet interface bilayers.Journal of the Royal Society, Interface · 2018Article
- Activation of the mechanosensitive ion channel MscL by mechanical stimulation of supported Droplet-Hydrogel bilayers.Scientific reports · 2017Article
- Engineering plant membranes using droplet interface bilayers.Biomicrofluidics · 2017Article
- In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers.Scientific reports · 2016Article
- Bilayer Networks within a Hydrogel Shell: A Robust Chassis for Artificial Cells and a Platform for Membrane Studies.Angewandte Chemie (International ed. in English) · 2016Article
- Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers.Journal of visualized experiments : JoVE · 2015Article
- A microfluidic platform for size-dependent generation of droplet interface bilayer networks on rails.Biomicrofluidics · 2015Article
- Activation of bacterial channel MscL in mechanically stimulated droplet interface bilayers.Scientific reports · 2015Article
- Microchamber device for detection of transporter activity of adherent cells.Frontiers in bioengineering and biotechnology · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Droplet interface bilayers (DIBs) provide an exciting new platform for the study of membrane proteins in stable bilayers of controlled composition. To date, the successful reconstitution and activity measurement of membrane proteins in DIBs has relied on the use of the synthetic lipid 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC). We report the functional reconstitution of the mechanosensitive channel of large conductance (MscL) into DIBs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), a lipid of significantly greater biological relevance than DPhPC. MscL functionality has been demonstrated using a fluorescence-based assay, showing that dye flow occurs across the DIB when MscL is gated by the cysteine reactive chemical 2-(trimethylammonium)ethyl methane thiosulfonate bromide (MTSET). MscL has already been the subject of a number of studies investigating its interaction with the membrane. We propose that this method will pave the way for future MscL studies looking in detail at the effects of controlled composition or membrane asymmetry on MscL activity using biologically relevant lipids and will also be applicable to other lipid-protein systems, paving the way for the study of membrane proteins in DIBs with biologically relevant lipids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.