Evidence map›Paper›PMID 25007843›Full record

ArticleBMC genomics2014

Genome and transcriptome sequencing identifies breeding targets in the orphan crop tef (Eragrostis tef).

Gina Cannarozzi, Sonia Plaza-Wüthrich, Korinna Esfeld, Stéphanie Larti, Yi Song Wilson, Dejene Girma, Edouard de Castro, Solomon Chanyalew, Regula Blösch, Laurent Farinelli and 6 more

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.

0numbers the graph read from it
0cells of the map it votes in
76citing papers in PubMed
15.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

76 citing papers in PubMed, 188 citations in OpenAlex.

  1. Article
  2. Article
  3. The genomics of t'ef and finger millet domestication and spread.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Article
  4. Millets for a sustainable future.Journal of experimental botany · 2025
    Review
  5. Article
  6. Article
  7. Review
  8. Genomic resources, opportunities, and prospects for accelerated improvement of millets.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
    Review
  9. Transcriptomic Profile of Tef (Plants (Basel, Switzerland) · 2024
    Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review

16 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 3 countries.

Gina Cannarozzi
Sonia Plaza-Wüthrich
Korinna Esfeld
Stéphanie Larti
Yi Song Wilson
Dejene Girma
Edouard de Castro
Solomon Chanyalew
Regula Blösch
Laurent Farinelli
Eric Lyons
Michel Schneider
Laurent Falquet
Cris Kuhlemeier
Kebebew Assefa
Zerihun TadeleUniversity of Bern, Institute of Plant Sciences, Altenbergrain 21, Bern CH-3013, Switzerland. zerihun.tadele@ips.unibe.ch.
University of Bern · CHSIB Swiss Institute of Bioinformatics · CHL.A.C. (Switzerland) · CHUniversity of Arizona · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTef (Eragrostis tef), an indigenous cereal critical to food security in the Horn of Africa, is rich in minerals and protein, resistant to many biotic and abiotic stresses and safe for diabetics as well as sufferers of immune reactions to wheat gluten. We present the genome of tef, the first species in the grass subfamily Chloridoideae and the first allotetraploid assembled de novo. We sequenced the tef genome for marker-assisted breeding, to shed light on the molecular mechanisms conferring tef's desirable nutritional and agronomic properties, and to make its genome publicly available as a community resource.

resultsThe draft genome contains 672 Mbp representing 87% of the genome size estimated from flow cytometry. We also sequenced two transcriptomes, one from a normalized RNA library and another from unnormalized RNASeq data. The normalized RNA library revealed around 38000 transcripts that were then annotated by the SwissProt group. The CoGe comparative genomics platform was used to compare the tef genome to other genomes, notably sorghum. Scaffolds comprising approximately half of the genome size were ordered by syntenic alignment to sorghum producing tef pseudo-chromosomes, which were sorted into A and B genomes as well as compared to the genetic map of tef. The draft genome was used to identify novel SSR markers, investigate target genes for abiotic stress resistance studies, and understand the evolution of the prolamin family of proteins that are responsible for the immune response to gluten.

conclusionsIt is highly plausible that breeding targets previously identified in other cereal crops will also be valuable breeding targets in tef. The draft genome and transcriptome will be of great use for identifying these targets for genetic improvement of this orphan crop that is vital for feeding 50 million people in the Horn of Africa.

Indexed as

Genome, PlantTranscriptomeChromosome MappingEragrostisGene LibraryHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsMolecular Sequence AnnotationPhosphoric Monoester HydrolasesPhylogenyPlant ProteinsProlaminsRNA, UntranslatedSequence Analysis, RNAPhosphoric Monoester HydrolasesPlant ProteinsProlaminsRNA, Untranslated

Identifiers

PMID25007843
PMCPMC4119204
OpenAlexW2160048049

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.