ArticleInternational journal of molecular sciences2014
Development of an intergeneric conjugal transfer system for xinaomycins-producing Streptomyces noursei Xinao-4.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Fluorescence-Based Soil Survival Analysis of the Xenobiotic- and Metal-DetoxifyingInternational journal of molecular sciences · 2025Article
- Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids.Applied microbiology and biotechnology · 2025Article
- Efficacy and Molecular Mechanisms of Nystatin AgainstFoods (Basel, Switzerland) · 2024Article
- Enhanced ε-Poly-L-Lysine Production inBiomolecules · 2024Article
- Recent Advances in Chemical Synthesis of Amino Sugars.Molecules (Basel, Switzerland) · 2023Review
- Development of a thiostrepton-free system for stable production of PLD in Streptomyces lividans SBT5.Microbial cell factories · 2022Article
- An Efficient Method To Generate Gene Deletion Mutants of the Rapamycin-Producing Bacterium Streptomyces iranensis HM 35.Applied and environmental microbiology · 2016Article
- Development of an efficient electroporation method for iturin A-producing Bacillus subtilis ZK.International journal of molecular sciences · 2015Article
- Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527.Journal of Zhejiang University. Science. BArticle
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5 authors.
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Abstract
To introduce DNA into Streptomyces noursei xinao-4, which produces xinaomycins, we explored an intergeneric conjugal transfer system. High efficiency of conjugation (8×10(-3) exconjugants per recipient) was obtained when spores of S. noursei xinao-4 were heat-shocked at 50 °C for 10 min, mixed with Escherichia coli ET12567 (pUZ8002/pSET152) in the ratio of 1:100, plated on 2CMY medium containing 40 mmol/L MgCl2, and incubated at 30 °C for 22 h. With this protocol, the plasmids pKC1139 and pSET152 were successfully transferred from E. coli ET12567 (pUZ8002) with different frequencies. Among all parameters, the ratio of donor to recipient cell number had the strongest effect on the transformation efficiency. In order to validate the above intergeneric conjugal transfer system, a glycosyltransferase gene was cloned and efficiently knocked out in S. noursei xinao-4 using pSG5-based plasmid pKC1139.
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