ArticlePloS one2014
A comparison of loop-mediated isothermal amplification (LAMP) with other surveillance tools for Echinococcus granulosus diagnosis in canine definitive hosts.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Meta-analysis of the prevalence of Echinococcus in dogs in China from 2010 to 2019.PLoS neglected tropical diseases · 2021Pooled it
- Ultrasensitive, low-input detection of avocado sunblotch viroid via RPA-CRISPR and nanopore-array single-bead fluorescence readout.Microsystems & nanoengineering · 2026Article
- CRISPR/Cas12a-RCA enables ultrasensitive detection of circulating free DNA for noninvasive diagnosis of echinococcosis.PLoS neglected tropical diseases · 2026Article
- Echinococcus granulosus sensu lato control measures: a specific focus on vaccines for both definitive and intermediate hosts.Parasites & vectors · 2024Review
- LAMP Assay for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- LAMP in Neglected Tropical Diseases: A Focus on Parasites.Diagnostics (Basel, Switzerland) · 2021Review
- An accurate, rapid and simple loop-mediated isothermal amplification method forVeterinary research forum : an international quarterly journal · 2021Article
- Development of a New LAMP Assay for the Detection ofFrontiers in veterinary science · 2021Article
- Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections.International journal of molecular sciences · 2020Review
- Currently Available Monitoring and Surveillance Systems forPathogens (Basel, Switzerland) · 2020Review
- Detection of helminths by loop-mediated isothermal amplification assay: a review of updated technology and future outlook.Infectious diseases of poverty · 2019Review
- Echinococcosis: Advances in the 21st Century.Clinical microbiology reviews · 2019Review
- Estimating the prevalence of Echinococcus in domestic dogs in highly endemic for echinococcosis.Infectious diseases of poverty · 2018Article
- Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area.The Journal of veterinary medical science · 2017Article
- Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis.BMC veterinary research · 2016Article
- Risk factors for Echinococcus coproantigen positivity in dogs from the Alay valley, Kyrgyzstan.Journal of helminthology · 2015Article
- Detection of SpikedIranian journal of parasitologyArticle
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Authors and funding
17 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCystic echinococcosis is highly prevalent in northwest China. A cost-effective, easy to operate diagnostic tool with high sensitivity and specificity would greatly facilitate the monitoring of Echinococcus infections in canine definitive hosts.
methodsThe primers used in the LAMP assay were based on the mitochondrial nad5 gene of E. granulosus sensu stricto (E. granulosus s.s., or E.g.s.s.) and were designed using Primer Explorer V4 software. The developed LAMP assay was compared with a conventional PCR method, copro-ELISA and microscopy, using the faeces of dogs experimentally infected with E.g.s.s., and field-collected faeces of domestic dogs including 190 from Qinghai province highly endemic for E.g.s.s. and 30 controls from an area in Gansu, where a domestic dog de-worming program was in operation.
resultsThe positivity rates obtained for the field-collected faecal samples were 12.6%, 1.6% and 2.1% by the LAMP, PCR and copro-ELISA assays, respectively. All samples obtained from the control dogs were negative. Compared with the conventional PCR, the LAMP assay provided 88.8% specificity and 100% sensitivity. The higher sensitivity of the LAMP method was also shown by the fact that it could detect the presence of laboratory challenge dog infections of E. granulsous s.s. four days earlier than the PCR method. Three copro-samples shown positive by the commercial copro-ELISA were all negative by LAMP, PCR and microscopy, which suggests these samples may have originated from another infection rather than E. granulsous s.s., possibly E. shiquicus or E. Canadensis, which is also present in China.
conclusionsWe have developed a potentially useful surveillance tool for determining the prevalence of canine E. granulosus s.s. infections in the field. The LAMP assay may lead to a more cost-effective and practicable way of tracking Echinococcus infections in canids, especially when combined with the copro-ELISA.
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