ArticleNature communications2014
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.
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Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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102citing papers in PubMed
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Who cites it
102 citing papers in PubMed, 230 citations in OpenAlex.
- The structure of intact and active Photosystem II from Arabidopsis thaliana at 2.44 Å resolution.The New phytologist · 2026Article
- Sensitive detection of structural dynamics using a statistical framework for comparative crystallography.Science advances · 2025Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- An analysis of the structural changes of the oxygen evolving complex of Photosystem II in the SBiochimica et biophysica acta. Bioenergetics · 2025Review
- Cryo-EM meets crystallography: A model-independent view of the heteronuclear MnProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lighting the way: Compelling open questions in photosynthesis research.The Plant cell · 2024Article
- Innovative Strategies in X-ray Crystallography for Exploring Structural Dynamics and Reaction Mechanisms in Metabolic Disorders.Journal of personalized medicine · 2024Review
- Sensitive Detection of Structural Differences using a Statistical Framework for Comparative Crystallography.bioRxiv : the preprint server for biology · 2024Article
- Structure Function Studies of Photosystem II Using X-Ray Free Electron Lasers.Annual review of biophysics · 2024Review
- Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels.The Journal of biological chemistry · 2024Article
- Experimental capabilities for liquid jet samples at sub-MHz rates at the FXE Instrument at European XFEL.Journal of synchrotron radiation · 2023Article
- Combining on-line spectroscopy with synchrotron and X-ray free electron laser crystallography.Current opinion in structural biology · 2023Review
- Does Serial Femtosecond Crystallography Depict State-Specific Catalytic Intermediates of the Oxygen-Evolving Complex?Journal of the American Chemical Society · 2023Article
- X-ray Emission Spectroscopy of Single Protein Crystals Yields Insights into Heme Enzyme Intermediates.The journal of physical chemistry letters · 2023Article
- A unifying Bayesian framework for merging X-ray diffraction data.Nature communications · 2022Article
- Electrically stimulated droplet injector for reduced sample consumption in serial crystallography.Biophysical reports · 2022Article
- Competitive interaction of Mn(II) and Fe(II) cations with the high-affinity Mn-binding site of the photosystem II: evolutionary aspect.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2022Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- Hole Dynamics in Photoexcited Hematite Studied with Femtosecond Oxygen K-edge X-ray Absorption Spectroscopy.The journal of physical chemistry letters · 2022Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
42 authors at 9 institutions in 4 countries.
Jan Kern1] Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA [2] LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Rosalie TranPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Roberto Alonso-MoriLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Sergey KoroidovInstitutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, 90187 Umeå, Sweden.
Nathaniel EcholsPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Johan HattnePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Mohamed Ibrahim1] Institut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany [2] Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany.
Sheraz GulPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Hartawan LaksmonoPULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Raymond G SierraPULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Richard J Gildea1] Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA [2].
Guangye HanPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Julia Hellmich1] Institut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany [2] Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany.
Benedikt Lassalle-Kaiser1] Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA [2].
Ruchira ChatterjeePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Aaron S BrewsterPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Claudiu A StanPULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Carina GlöcknerMax-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany.
Alyssa LampePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Dörte DiFioreMax-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany.
Despina MilathianakiLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Alan R FryLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
M Marvin Seibert1] LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA [2].
Jason E KoglinLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Erik GalloEuropean Synchrotron Radiation Facility, F-38043 Grenoble, France.
Jens UhligEuropean Synchrotron Radiation Facility, F-38043 Grenoble, France.
Dimosthenis SokarasSSRL, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Tsu-Chien WengSSRL, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Petrus H ZwartPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
David E SkinnerNational Energy Research Scientific Computing Center, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Michael J Bogan1] LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA [2] PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Marc MesserschmidtLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Pieter GlatzelEuropean Synchrotron Radiation Facility, F-38043 Grenoble, France.
Garth J WilliamsLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Sébastien BoutetLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Paul D AdamsPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Athina Zouni1] Institut für Biologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany [2] Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, D-10623 Berlin, Germany.
Johannes MessingerInstitutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, 90187 Umeå, Sweden.
Nicholas K SauterPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Uwe BergmannLCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA.
Junko YanoPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Vittal K YachandraPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Lawrence Berkeley National Laboratory · USStanford University · USSLAC National Accelerator Laboratory · USEuropean Synchrotron Radiation Facility · FRTechnische Universität Berlin · DEHumboldt-Universität zu Berlin · DEStanford Synchrotron Radiation Lightsource · USUmeå University · SENational Energy Research Scientific Computing Center · US
Funding
PROJECT 5 - DUKE - STRUCTURE VALIDATION AND IMPROVEMENT FOR PROTEINS AND N. ACIDSP01GM063210 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI ADAMS, PAUL DAVID · 2001 to 2021
$33.5MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MTaking Snapshots of the Water-Oxidation Reaction by the Mn4Ca Oxygen-Evolving Complex Using X-ray Crystallography and SpectroscopyR01GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 1997 to 2022
$9.1MBiological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8MRealizing New Horizons in X-ray Crystallography Data ProcessingR01GM095887 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2011 to 2014
$1.5MTowards real-time XFEL data reduction with CCTBXR01GM102520 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2012 to 2015
$1.4MStructure and Function of the Mn Oxygen-Evolving ComplexR56GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 2009 to 2009
$530kNIGMS NIH HHS GM055302NIGMS NIH HHS GM095887NIGMS NIH HHS GM102520NIGMS NIH HHS P01 GM063210NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41GM103393NIGMS NIH HHS R01 GM055302NIGMS NIH HHS R01 GM095887NIGMS NIH HHS R01 GM102520NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R56 GM055302
Abstract
The dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II.
Indexed as
CyanobacteriaModels, ChemicalOxidation-ReductionOxygenPhotosynthesisPhotosystem II Protein ComplexSpectrometry, X-Ray EmissionWaterX-Ray DiffractionOxygenPhotosystem II Protein ComplexWater
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