ArticleJournal of experimental botany2014
A high throughput gas exchange screen for determining rates of photorespiration or regulation of C4 activity.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Rapid dehydration drives a nondiffusional drop in CThe New phytologist · 2026Article
- Modeling with uncertainty quantification reveals the essentials of a non-canonical algal carbon-concentrating mechanism.Plant physiology · 2025Article
- Fast dehydration reduces bundle sheath conductance in CThe New phytologist · 2024Article
- Tomato and mini-cucumber tolerance to photoperiodic injury involves photorespiration and the engagement of nighttime cyclic electron flow from dynamic LEDs.Frontiers in plant science · 2024Article
- Reduction of bundle sheath size boosts cyclic electron flow in CThe Plant journal : for cell and molecular biology · 2022Article
- Photoprotection Is Achieved by Photorespiration and Modification of the Leaf Incident Light, and Their Extent Is Modulated by the Stomatal Sensitivity to Water Deficit in Grapevines.Plants (Basel, Switzerland) · 2022Article
- Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves.Metabolic engineering · 2022Article
- Storing carbon in leaf lipid sinks enhances perennial ryegrass carbon capture especially under high N and elevated CO2.Journal of experimental botany · 2020Article
- Response of photosynthesis, growth and water relations of a savannah-adapted tree and grass grown across high to low CO2.Annals of botany · 2019Article
- Estimating CPhotosynthesis research · 2019Article
- A generalised dynamic model of leaf-level CPhotosynthesis research · 2019Article
- Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis.The New phytologist · 2017Article
- A generalized stoichiometric model of C3, C2, C2+C4, and C4 photosynthetic metabolism.Journal of experimental botany · 2017Article
- Systems biology and metabolic modelling unveils limitations to polyhydroxybutyrate accumulation in sugarcane leaves; lessons for C4 engineering.Plant biotechnology journal · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Large-scale research programmes seeking to characterize the C4 pathway have a requirement for a simple, high throughput screen that quantifies photorespiratory activity in C3 and C4 model systems. At present, approaches rely on model-fitting to assimilatory responses (A/C i curves, PSII quantum yield) or real-time carbon isotope discrimination, which are complicated and time-consuming. Here we present a method, and the associated theory, to determine the effectiveness of the C4 carboxylation, carbon concentration mechanism (CCM) by assessing the responsiveness of V O/V C, the ratio of RuBisCO oxygenase to carboxylase activity, upon transfer to low O2. This determination compares concurrent gas exchange and pulse-modulated chlorophyll fluorescence under ambient and low O2, using widely available equipment. Run time for the procedure can take as little as 6 minutes if plants are pre-adapted. The responsiveness of V O/V C is derived for typical C3 (tobacco, rice, wheat) and C4 (maize, Miscanthus, cleome) plants, and compared with full C3 and C4 model systems. We also undertake sensitivity analyses to determine the impact of R LIGHT (respiration in the light) and the effectiveness of the light saturating pulse used by fluorescence systems. The results show that the method can readily resolve variations in photorespiratory activity between C3 and C4 plants and could be used to rapidly screen large numbers of mutants or transformants in high throughput studies.
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Registered trials
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