ArticlePloS one2014
miR-196b-mediated translation regulation of mouse insulin2 via the 5'UTR.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- The diagnostic value of circulating microRNAs as biomarkers for coronary artery disease: A meta‑analysis.Anatolian journal of cardiology · 2020Pooled it
- The interaction between ΔNp63α and TAp63α, mediated by miR-205-5p, inhibits the migration of lung adenocarcinoma cells.Scientific reports · 2025Article
- Structural Features of 5' Untranslated Region in Translational Control of Eukaryotes.International journal of molecular sciences · 2025Review
- Differential microRNA expression patterns between TallyHo/JngJ mice and non-diabetic Swiss Webster Random/Jackson mice.JBMR plus · 2025Article
- A novel upregulated hsa_circ_0032746 regulates the oncogenesis of esophageal squamous cell carcinoma by regulating miR-4270/MCM3 axis.Human genomics · 2024Article
- MiR-4492, a New Potential MicroRNA for Cancer Diagnosis and Treatment: A Mini Review.Chonnam medical journal · 2024Review
- Decoding the genetic relationship between Alzheimer's disease and type 2 diabetes: potential risk variants and future direction for North Africa.Frontiers in aging neuroscience · 2023Article
- In-depth investigation of microRNA-mediated cross-kingdom regulation between Asian honey bee and microsporidian.Frontiers in microbiology · 2022Article
- Vitamin D deficiency inhibits microRNA-196b-5p which regulates ovarian granulosa cell hormone synthesis, proliferation, and apoptosis by targetingAnnals of translational medicine · 2021Article
- CircRNA: A novel potential strategy to treat thyroid cancer (Review).International journal of molecular medicine · 2021Review
- Ribosome Fingerprinting with a Solid-State Nanopore.ACS sensors · 2020Article
- Identification and Characterization of Circular Intronic RNAs Derived from Insulin Gene.International journal of molecular sciences · 2020Article
- Health effects of particulate matter air pollution in underground railway systems - a critical review of the evidence.Particle and fibre toxicology · 2019Review
- Regulatory network of miRNA on its target: coordination between transcriptional and post-transcriptional regulation of gene expression.Cellular and molecular life sciences : CMLS · 2019Review
- Reduced expression of the RNA-binding protein HuD in pancreatic neuroendocrine tumors correlates with low p27The Journal of pathology · 2018Article
- Interaction of HuDA and PABP at 5'UTR of mouse insulin2 regulates insulin biosynthesis.PloS one · 2018Article
- MicroRNA, Proteins, and Metabolites as Novel Biomarkers for Prediabetes, Diabetes, and Related Complications.Frontiers in endocrinology · 2018Review
- Regulation of Adult Neurogenesis by Non-coding RNAs: Implications for Substance Use Disorders.Frontiers in neuroscience · 2018Review
- SASP regulation by noncoding RNA.Mechanisms of ageing and development · 2017Review
- Polysome Fractionation to Analyze mRNA Distribution Profiles.Bio-protocol · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The 5' and the 3' untranslated regions (UTR) of the insulin genes are very well conserved across species. Although microRNAs (miRNAs) are known to regulate insulin secretion process, direct regulation of insulin biosynthesis by miRNA has not been reported. Here, we show that mouse microRNA miR-196b can specifically target the 5'UTR of the long insulin2 splice isoform. Using reporter assays we show that miR-196b specifically increases the translation of the reporter protein luciferase. We further show that this translation activation is abolished when Argonaute 2 levels are knocked down after transfection with an Argonaute 2-directed siRNA. Binding of miR-196b to the target sequence in insulin 5'UTR causes the removal of HuD (a 5'UTR-associated translation inhibitor), suggesting that both miR-196b and HuD bind to the same RNA element. We present data suggesting that the RNA-binding protein HuD, which represses insulin translation, is displaced by miR-196b. Together, our findings identify a mechanism of post-transcriptional regulation of insulin biosynthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.