ArticlePloS one2014
Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed.
- Integrated Phenotypic, Proteomic (MALDI-TOF MS), and Genomic (WGS) Investigation of a Prolonged Hospital Outbreak ofAntibiotics (Basel, Switzerland) · 2026Article
- Integrated matrix-assisted laser desorption ionization time-of-flight mass spectrometry serotype-specific peak analysis andVeterinary world · 2026Article
- Epidemiological insights into virulence and antimicrobial resistance ofFrontiers in veterinary science · 2026Article
- Haemophilus influenzae and Staphylococcus aureus population shifts during social distancing as monitored by MALDI-TOF MS.Scientific reports · 2025Article
- Metabolites derived from bacterial isolates of the human skin microbiome inhibitMicrobiology spectrum · 2025Article
- Typing of clinical and reference strains of Saccharomyces cerevisiae using pulsed-field gel electrophoresis and MALDI-TOF MS.Scientific reports · 2025Article
- Contribution of MALDI-TOF mass spectrometry and machine learning including deep learning techniques for the detection of virulence factors of Clostridioides difficile strains.Microbial biotechnology · 2024Article
- Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Molecular Typing of Acinetobacter baumannii in Comparison with Orthogonal Methods.Microbiology spectrum · 2023Article
- Identification and Subtyping ofMicroorganisms · 2022Article
- Rapid Identification ofFrontiers in microbiology · 2022Article
- Using MALDI-TOF spectra in epidemiological surveillance for the detection of bacterial subgroups with a possible epidemic potential.BMC infectious diseases · 2021Article
- Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising?Current issues in molecular biology · 2021Review
- Exploring the value of MALDI-TOF MS for the detection of clonal outbreaks of Burkholderia contaminans.Journal of microbiological methods · 2021Article
- Article
- Factors Associated With MALDI-TOF Mass Spectral Quality of Species Identification in Clinical Routine Diagnostics.Frontiers in cellular and infection microbiology · 2021Article
- Determination of methicillin-resistantClinical mass spectrometry (Del Mar, Calif.) · 2020Article
- Putative Protein Biomarkers ofBiology · 2020Article
- Insights Into Subspecies Discrimination Potentiality From Bacteria MALDI-TOF Mass Spectra by Using Data Mining and Diversity Studies.Frontiers in microbiology · 2020Article
- Proteotyping as alternate typing method to differentiate Campylobacter coli clades.Scientific reports · 2019Article
- Review of the impact of MALDI-TOF MS in public health and hospital hygiene, 2018.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have therefore evaluated the applicability of a MALDI-TOF mass spectrometry based strategy for outbreak strain identification.
methodsSpecific peaks in the outbreak strain's spectrum were identified by comparative analysis of archived pre-outbreak spectra that had been acquired for routine species-level identification. Proteins underlying these discriminatory peaks were identified by liquid chromatography tandem mass spectrometry and validated against publicly available databases. The resulting typing scheme was evaluated against PCR genotyping with 294 E. coli isolates from clinical samples collected during the outbreak.
resultsComparative spectrum analysis revealed two characteristic peaks at m/z 6711 and m/z 10883. The underlying proteins were found to be of low prevalence among genome sequenced E. coli strains. Marker peak detection correctly classified 292 of 293 study isolates, including all 104 outbreak isolates.
conclusionsMALDI-TOF mass spectrometry allowed for reliable outbreak strain identification during a large outbreak of Shiga-Toxigenic E. coli. The applied typing strategy could probably be adapted to other typing tasks and might facilitate epidemiological surveys as part of the routine pathogen identification workflow.
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