Evidence map›Paper›PMID 25003339›Full record

ArticlePloS one2014

Immunomodulatory effects of bone marrow-derived mesenchymal stem cells on pro-inflammatory cytokine-stimulated human corneal epithelial cells.

Li Wen, Meidong Zhu, Michele C Madigan, Jingjing You, Nicholas J C King, Francis A Billson, Kathryn McClellan, Gerard Sutton, Con Petsoglou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Concise Review: Bioengineering of Limbal Stem Cell Niche.Bioengineering (Basel, Switzerland) · 2023
    Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021
    Review
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
  15. Effects of retinoic acid receptor-γ on theInternational journal of ophthalmology · 2016
    Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Li WenSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia.
Meidong ZhuSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; Lions New South Wales Eye Bank, NSW Organ and Tissue Donation Service, Sydney, New South Wales, Australia; Sydney Eye Hospital, Sydney, New South Wales, Australia.
Michele C MadiganSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; School of Optometry & Vision Science, University of New South Wales, Kensington, New South Wales, Australia.
Jingjing YouSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia.
Nicholas J C KingDiscipline of Pathology, School of Medical Sciences and Bosch Institute, Sydney Medical School, University of Sydney, Camperdown, New South Wales, Australia.
Francis A BillsonSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; Sydney Eye Hospital, Sydney, New South Wales, Australia; Sight for Life Foundation, Sydney, New South Wales, Australia.
Kathryn McClellanSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; Lions New South Wales Eye Bank, NSW Organ and Tissue Donation Service, Sydney, New South Wales, Australia; Sydney Eye Hospital, Sydney, New South Wales, Australia.
Gerard SuttonSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; Lions New South Wales Eye Bank, NSW Organ and Tissue Donation Service, Sydney, New South Wales, Australia; Sydney Eye Hospital, Sydney, New South Wales, Australia; Vision Eye Institute, Chatswood, New South Wales, Australia; Auckland University, Auckland, New Zealand.
Con PetsoglouSave Sight Institute & Discipline of Clinical Ophthalmology, University of Sydney, Sydney, New South Wales, Australia; Lions New South Wales Eye Bank, NSW Organ and Tissue Donation Service, Sydney, New South Wales, Australia; Sydney Eye Hospital, Sydney, New South Wales, Australia.
University of Sydney · AUQuest for Life Foundation · AUSydney Hospital · AUUniversity of Auckland · NZUNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate the modulatory effect of rat bone marrow mesenchymal stem cells (MSC) on human corneal epithelial cells (HCE-T) stimulated with pro-inflammatory cytokines interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) in an in vitro co-cultured model.

methodsHCE-T alone and co-cultured with MSC were stimulated with IFN-γ/TNF for 24 and 48 hours or left untreated. The expression of intracellular adhesion molecule (ICAM)-1, human leukocyte antigen ABC, DR and G (HLA-ABC, HLA-DR, HLA-G) were investigated by flow cytometry. Subcellular localization of nuclear factor-kappa B (NF-κB) and expression of indoleamine 2,3-dioxygenase (IDO) were assessed by immunofluorescence staining and western blot. The concentration of transforming growth factor beta 1 (TGF-β1) in the conditioned media from different cultures was evaluated by enzyme-linked immunosorbent assay. NF-κB and TGF-β1 signaling pathway blocking experiments were performed to analyze associations between the expression of cell surface molecules and the NF-κB transcription pathway, and the expression of IDO and TGF-β1 signaling pathway.

resultsIFN-γ/TNF treatment significantly up-regulated expression of ICAM-1, HLA-ABC, and induced de novo expression of HLA-DR and IDO on HCE-T cultured alone, while HLA-G expression remained unaffected. Up-regulation was significantly inhibited by co-culture with MSC. Increased TGF-β1 secretion was detected in 48 h IFN-γ/TNF-stimulated MSC monocultures and HCE-T/MSC co-cultures. MSC attenuated the activation of cytokine-induced NF-κB and IDO induction. Blockade of NF-κB transcription pathway by BMS-345541 significantly reduced the up-regulation of ICAM-1, HLA-ABC, HLA-DR and IDO expression, while blockade of TGF-β1 signaling pathways reversed the modulatory effect of MSC on IDO expression.

conclusionsMSC reduced the expression of adhesion and immunoregulatory molecules on pro-inflammatory cytokine-stimulated HCE-T via the NF-κB transcription pathway. MSC attenuated expression of IDO through both NF-κB transcription and TGF-β1 signaling pathways. Co-culture of HCEC with MSC therefore provides a useful in vitro model to study the anti-inflammatory properties of MSC on corneal epithelium.

Indexed as

AnimalsCell Adhesion MoleculesCell Line, TransformedCytokinesEnzyme ActivationEpithelial CellsEpithelium, CornealHumansImmunomodulationImmunophenotypingIndoleamine-Pyrrole 2,3,-DioxygenaseInflammation MediatorsMesenchymal Stem CellsNF-kappa BPhenotypeProtein TransportCell Adhesion MoleculesCytokinesIndoleamine-Pyrrole 2,3,-DioxygenaseInflammation MediatorsNF-kappa BTransforming Growth Factor beta1

Identifiers

PMID25003339
PMCPMC4086952
OpenAlexW2045588888

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.