ArticlePloS one2014
Transgenic tobacco plants overexpressing a grass PpEXP1 gene exhibit enhanced tolerance to heat stress.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 89 citations in OpenAlex.
- Design Principles of Plant Thermotolerance: Integrating Photosynthesis, Reproduction, and Field Resilience in a Warming Climate.Plant, cell & environment · 2026Review
- An integrated WGCNA-XGBoost framework for prioritizing candidate stress-responsive hub genes in soybean under nutrient and heavy metal stress conditions.Journal of applied genetics · 2026Article
- Genome-Wide Characterization of thePlants (Basel, Switzerland) · 2026Article
- Decoding core molecular mechanisms of heat-stress tolerance in Brassica napus using transcriptomics and machine learning.Plant cell reports · 2026Article
- Identification of HSF gene family and functional analysis of two HSFA1 genes with tandem repeat features in Fritillaria cirrhosa D.Don.BMC genomics · 2026Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Wheat COBRA-like GeneInternational journal of molecular sciences · 2025Article
- Effect of Short-Term High-COPlants (Basel, Switzerland) · 2024Article
- Function Analysis of a Maize Endo-1,4-β-xylanase GeneInternational journal of molecular sciences · 2024Article
- Alpha-expansins: more than three decades of wall creep and loosening in fruits.Plant molecular biology · 2024Review
- Intraspecific variation in responses to extreme and moderate temperature stress in the wild species,AoB PLANTS · 2024Article
- Identification of genes associated with abiotic stress tolerance in sweetpotato using weighted gene co-expression network analysis.Plant direct · 2023Article
- Evolution and Expression of the Expansin Genes in Emmer Wheat.International journal of molecular sciences · 2023Article
- Comprehensive Transcriptome Profiling Uncovers Molecular Mechanisms and Potential Candidate Genes Associated with Heat Stress Response in Chickpea.International journal of molecular sciences · 2023Article
- Characterization of the Cell Wall Component through Thermogravimetric Analysis and Its Relationship with an Expansin-like Protein inInternational journal of molecular sciences · 2022Article
- Genome-wide identification, characterization of expansin gene family of banana and their expression pattern under various stresses.3 Biotech · 2022Article
- Molecular insights into sensing, regulation and improving of heat tolerance in plants.Plant cell reports · 2022Review
- Comparative Phenotyping of Two Commonly UsedPlants (Basel, Switzerland) · 2022Article
- Comprehensive transcriptome analysis reveals heat-responsive genes in flowering Chinese cabbage (Frontiers in plant science · 2022Article
- Water Stress Differentially Modulates the Expression of Tomato Cell Wall Metabolism-Related Genes inFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat stress is a detrimental abiotic stress limiting the growth of many plant species and is associated with various cellular and physiological damages. Expansins are a family of proteins which are known to play roles in regulating cell wall elongation and expansion, as well as other growth and developmental processes. The in vitro roles of expansins regulating plant heat tolerance are not well understood. The objectives of this study were to isolate and clone an expansin gene in a perennial grass species (Poa pratensis) and to determine whether over-expression of expansin may improve plant heat tolerance. Tobacco (Nicotiana tabacum) was used as the model plant for gene transformation and an expansin gene PpEXP1 from Poa pratensis was cloned. Sequence analysis showed PpEXP1 belonged to α-expansins and was closely related to two expansin genes in other perennial grass species (Festuca pratensis and Agrostis stolonifera) as well as Triticum aestivum, Oryza sativa, and Brachypodium distachyon. Transgenic tobacco plants over-expressing PpEXP1 were generated through Agrobacterium-mediated transformation. Under heat stress (42°C) in growth chambers, transgenic tobacco plants over-expressing the PpEXP1 gene exhibited a less structural damage to cells, lower electrolyte leakage, lower levels of membrane lipid peroxidation, and lower content of hydrogen peroxide, as well as higher chlorophyll content, net photosynthetic rate, relative water content, activity of antioxidant enzyme, and seed germination rates, compared to the wild-type plants. These results demonstrated the positive roles of PpEXP1 in enhancing plant tolerance to heat stress and the possibility of using expansins for genetic modification of cool-season perennial grasses in the development of heat-tolerant germplasm and cultivars.
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