Evidence map›Paper›PMID 25003092›Full record

ArticleClinical and experimental vaccine research2014

Cellular immunity survey against urinary tract infection using pVAX/fimH cassette with mammalian and wild type codon usage as a DNA vaccine.

Abbas Ali Imani Fooladi, Ghasem Bagherpour, Nima Khoramabadi, Jalil Fallah Mehrabadi, Mehdi Mahdavi, Raheleh Halabian, Mohsen Amin, Jalal Izadi Mobarakeh, Behzad Einollahi

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and experimental vaccine research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Staphylococcal enterotoxin B as DNA vaccine against breast cancer in a murine model.International microbiology : the official journal of the Spanish Society for Microbiology · 2023
    Article
  3. Urinary Tract Infections Caused by UropathogenicInternational journal of molecular sciences · 2023
    Review
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Abbas Ali Imani FooladiApplied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Ghasem BagherpourApplied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Nima KhoramabadiBacteriology Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Jalil Fallah MehrabadiDepartment of Genetic Engineering, Faculty of Biosciences and Biotechnology, Malekashtar University of Technology, Tehran, Iran.
Mehdi MahdaviDepartment of Virology, Pasteur Institute of Iran, Tehran, Iran.
Raheleh HalabianApplied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Mohsen AminDepartment of Drug and Food Control, Tehran University of Medical Sciences, Tehran, Iran.
Jalal Izadi MobarakehDepartment of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.
Behzad EinollahiNephrology and Urology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Baqiyatallah University of Medical Sciences · IRPasteur Institute of Iran · IRMalek Ashtar University of Technology · IRTarbiat Modares University · IRTehran University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeFimH (the adhesion fragment of type 1 fimbriae) is implicated in uropathogenic Escherichia coli (UPEC) attachment to epithelial cells through interaction with mannose. Recently, some studies have found that UPEC can thrive intracellularly causing recurrent urinary tract infection (UTI). Almost all vaccines have been designed to induce antibodies against UPEC. Yet, the humoral immune response is not potent enough to overcome neither the primary UTI nor recurrent infections. However, DNA vaccines offer the possibility of inducing cell mediated immune responses and may be a promising preventive tool. MATERIALS AND

methodsIn this study, we employed two different open reading frames within mammalian (mam) and wild type (wt) codons of fimH gene. Optimized fragments were cloned in pVAX-1. Expression of the protein in COS-7 was confirmed by western blot analysis after assessing pVAX/fimH(mam) and pVAX/fimH(wt). The constructs were injected to BALB/c mice at plantar surface of feet followed by electroporation.

resultsThe mice immunized with both constructs following booster injection with recombinant FimH showed increased interferon-γ and interleukin-12 responses significantly higher than non-immunized ones (p<0.05). The immunized mice were challenged with UPEC and then the number of bacteria recovered from the immunized mice was compared with the non-immunized ones. Decreased colony count in immunized mice along with cytokine responses confirmed the promising immune response by the DNA vaccines developed in this study.

conclusionIn conclusion, DNA vaccines of UPEC proteins may confer some levels of protection which can be improved by multiple constructs or boosters.

Indexed as

Codon usage optimizationDNA vaccinesFimHUrinary tract infections

Identifiers

PMID25003092
PMCPMC4083071
OpenAlexW2048971809

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.