ArticleJournal of bacteriology2014
Evidence for a hexaheteromeric methylenetetrahydrofolate reductase in Moorella thermoacetica.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 101 citations in OpenAlex.
- Methanol Metabolism in the Cytochrome and Menaquinone-Containing Acetogen Moorella thermoacetica.Environmental microbiology · 2026Article
- Genomic and Transcriptomic Insights Into the Autotrophic Metabolism on HEnvironmental microbiology · 2026Article
- 5,10-Methylenetetrahydrofolate Reductase─the Key Allosteric Regulator in One-Carbon Metabolism.Biochemistry · 2026Review
- Methanol and Carbon Monoxide Metabolism of the Thermophile Moorella caeni.Environmental microbiology · 2025Article
- Article
- Mutational and structural studies of (βα)Protein science : a publication of the Protein Society · 2024Article
- The energy-converting hydrogenase Ech2 is important for the growth of the thermophilic acetogenMicrobiology spectrum · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- A diversified, widespread microbial gene cluster encodes homologs of methyltransferases involved in methanogenesis.bioRxiv : the preprint server for biology · 2023Article
- Genomic Insights into Syntrophic Lifestyle of 'Life (Basel, Switzerland) · 2023Article
- Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.Bioresources and bioprocessing · 2023Review
- Biosynthesis of L-5-methyltetrahydrofolate by genetically engineered Escherichia coli.Microbial biotechnology · 2022Article
- Deep-branching acetogens in serpentinized subsurface fluids of Oman.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Third Way of Energy Conservation in Acetogenic Bacteria.Microbiology spectrum · 2022Article
- Article
- Systems Biology on Acetogenic Bacteria for Utilizing C1 Feedstocks.Advances in biochemical engineering/biotechnology · 2022Article
- Is reduced ferredoxin the physiological electron donor for MetVF-type methylenetetrahydrofolate reductases in acetogenesis? A hypothesis.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.PLoS biology · 2022Review
- Engineering Acetogenic Bacteria for Efficient One-Carbon Utilization.Frontiers in microbiology · 2022Review
- Energy conservation under extreme energy limitation: the role of cytochromes and quinones in acetogenic bacteria.Extremophiles : life under extreme conditions · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Moorella thermoacetica can grow with H₂ and CO₂, forming acetic acid from 2 CO₂ via the Wood-Ljungdahl pathway. All enzymes involved in this pathway have been characterized to date, except for methylenetetrahydrofolate reductase (MetF). We report here that the M. thermoacetica gene that putatively encodes this enzyme, metF, is part of a transcription unit also containing the genes hdrCBA, mvhD, and metV. MetF copurified with the other five proteins encoded in the unit in a hexaheteromeric complex with an apparent molecular mass in the 320-kDa range. The 40-fold-enriched preparation contained per mg protein 3.1 nmol flavin adenine dinucleotide (FAD), 3.4 nmol flavin mononucleotide (FMN), and 110 nmol iron, almost as predicted from the primary structure of the six subunits. It catalyzed the reduction of methylenetetrahydrofolate with reduced benzyl viologen but not with NAD(P)H in either the absence or presence of oxidized ferredoxin. It also catalyzed the reversible reduction of benzyl viologen with NADH (diaphorase activity). Heterologous expression of the metF gene in Escherichia coli revealed that the subunit MetF contains one FMN rather than FAD. MetF exhibited 70-fold-higher methylenetetrahydrofolate reductase activity with benzyl viologen when produced together with MetV, which in part shows sequence similarity to MetF. Heterologously produced HdrA contained 2 FADs and had NAD-specific diaphorase activity. Our results suggested that the physiological electron donor for methylenetetrahydrofolate reduction in M. thermoacetica is NADH and that the exergonic reduction of methylenetetrahydrofolate with NADH is coupled via flavin-based electron bifurcation with the endergonic reduction of an electron acceptor, whose identity remains unknown.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.