ArticleJournal of cell science2014
Palmitoylation of plakophilin is required for desmosome assembly.
Article in Journal of cell science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Plakoglobin phosphorylation at serine 665 is capable of stabilizing cadherin-mediated adhesion in keratinocytes.JCI insight · 2026Article
- zDHHC-Mediated S-Palmitoylation in Skin Health and Its Targeting as a Treatment Perspective.International journal of molecular sciences · 2025Review
- The desmosome comes into focus.The Journal of cell biology · 2024Review
- Desmosomes at a glance.Journal of cell science · 2024Review
- Plakophilin-3 Binds the Membrane and Filamentous Actin without Bundling F-Actin.International journal of molecular sciences · 2023Article
- Epithelial-mesenchymal transition: The history, regulatory mechanism, and cancer therapeutic opportunities.MedComm · 2022Review
- Desmosomal Cadherins in Health and Disease.Annual review of pathology · 2022Review
- Plakophilin 3 and Par3 facilitate desmosomes' association with the apical junctional complex.Molecular biology of the cell · 2021Article
- A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid Modifications.Chemical reviews · 2021Review
- Global identification of S-palmitoylated proteins and detection of palmitoylating (DHHC) enzymes in heart.Journal of molecular and cellular cardiology · 2021Article
- The desmosome as a model for lipid raft driven membrane domain organization.Biochimica et biophysica acta. Biomembranes · 2020Review
- miRNA- and cytokine-associated extracellular vesicles mediate squamous cell carcinomas.Journal of extracellular vesicles · 2020Article
- Proteomic analysis of desmosomes reveals novel components required for epidermal integrity.Molecular biology of the cell · 2020Article
- S-acylated Golga7b stabilises DHHC5 at the plasma membrane to regulate cell adhesion.EMBO reports · 2019Article
- Depletion of the cellular cholesterol content reduces the dynamics of desmosomal cadherins and interferes with desmosomal strength.Histochemistry and cell biology · 2019Article
- The desmosome is a mesoscale lipid raft-like membrane domain.Molecular biology of the cell · 2019Article
- Keratins Regulate p38MAPK-Dependent Desmoglein Binding Properties in Pemphigus.Frontiers in immunology · 2018Article
- Palmitoylation of Desmoglein 2 Is a Regulator of Assembly Dynamics and Protein Turnover.The Journal of biological chemistry · 2016Article
- Palmitoylation of Progressive Rod-Cone Degeneration (PRCD) Regulates Protein Stability and Localization.The Journal of biological chemistry · 2016Article
- Desmosomes in acquired disease.Cell and tissue research · 2015Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Desmosomes are prominent adhesive junctions found in various epithelial tissues. The cytoplasmic domains of desmosomal cadherins interact with a host of desmosomal plaque proteins, including plakophilins, plakoglobin and desmoplakin, which, in turn, recruit the intermediate filament cytoskeleton to sites of cell-cell contact. Although the individual components of the desmosome are known, mechanisms regulating the assembly of this junction are poorly understood. Protein palmitoylation is a posttranslational lipid modification that plays an important role in protein trafficking and function. Here, we demonstrate that multiple desmosomal components are palmitoylated in vivo. Pharmacologic inhibition of palmitoylation disrupts desmosome assembly at cell-cell borders. We mapped the site of plakophilin palmitoylation to a conserved cysteine residue present in the armadillo repeat domain. Mutation of this single cysteine residue prevents palmitoylation, disrupts plakophilin incorporation into the desmosomal plaque and prevents plakophilin-dependent desmosome assembly. Finally, plakophilin mutants unable to become palmitoylated act in a dominant-negative manner to disrupt proper localization of endogenous desmosome components and decrease desmosomal adhesion. Taken together, these data demonstrate that palmitoylation of desmosomal components is important for desmosome assembly and adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.