ArticlePloS one2014
MLST subtypes and population genetic structure of Cryptosporidium andersoni from dairy cattle and beef cattle in northeastern China's Heilongjiang Province.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- A retrospective epidemiological analysis of human Cryptosporidium infection in China during the past three decades (1987-2018).PLoS neglected tropical diseases · 2020Pooled it
- Occurrence and Genetic Diversity ofAnimals : an open access journal from MDPI · 2026Article
- Occurrence and Molecular Characterization ofMicroorganisms · 2025Article
- Article
- Prevalence and Molecular Characterization ofAnimals : an open access journal from MDPI · 2024Article
- Study on genetic characteristics of Cryptosporidium isolates and first report of C. parvum IIdA24G2 subtype in dairy cattle in China.Parasitology research · 2024Article
- Prevalence and molecular characterization of Cryptosporidium spp. in dairy and beef cattle in Shanxi, China.Parasitology research · 2023Article
- Occurrence and Molecular Characterization ofAnimals : an open access journal from MDPI · 2022Article
- An Update on ZoonoticAnimals : an open access journal from MDPI · 2021Review
- Prevalence and multilocus genotyping of Cryptosporidium spp. in cattle in Jiangxi Province, southeastern China.Parasitology research · 2021Article
- SubtypingMicroorganisms · 2020Article
- Molecular detection of Cryptosporidium and Enterocytozoon bieneusi in dairy calves and sika deer in four provinces in Northern China.Parasitology research · 2020Article
- Genetic characterization reveals evidence for an association between water contamination and zoonotic transmission of aFood and waterborne parasitology · 2019Article
- Molecular characterization and distribution of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi from yaks in Tibet, China.BMC veterinary research · 2019Article
- Global Cryptosporidium Loads from Livestock Manure.Environmental science & technology · 2017Article
- Molecular Epidemiology of Cryptosporidiosis in China.Frontiers in microbiology · 2017Review
- Epidemiology of Cryptosporidium infection in cattle in China: a review.Parasite (Paris, France) · 2017Review
- The first report of Cryptosporidium andersoni in horses with diarrhea and multilocus subtype analysis.Parasites & vectors · 2015Article
- Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle, northwest China.Parasitology research · 2015Article
- Molecular epidemiologic tools for waterborne pathogensFood and waterborne parasitologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cattle are the main reservoir host of C. andersoni, which shows a predominance in yearlings and adults of cattle. To understand the subtypes of C. andersoni and the population genetic structure in Heilongjiang Province, fecal specimens were collected from 420 dairy cattle and 405 beef cattle at the age of 12-14 months in eight cattle farms in five areas within this province and were screened for the presence of Cryptosporidium oocysts by microscopy after Sheather's sugar flotation technique. The average prevalence of Cryptosporidium spp. was 19.15% (158/825) and all the Cryptosporidium isolates were identified as C. andersoni by the SSU rRNA gene nested PCR-RFLP using SspI, VspI and MboII restriction enzymes. A total of 50 C. andersoni isolates were randomly selected and sequenced to confirm the RFLP results before they were subtyped by multilocus sequence typing (MLST) at the four microsatellite/minisatellite loci (MS1, MS2, MS3 and MS16). Four, one, two and one haplotypes were obtained at the four loci, respectively. The MLST subtype A4,A4,A4,A1 showed an absolute predominance and a wide distribution among the six MLST subtypes obtained in the investigated areas. Linkage disequilibrium analysis showed the presence of a clonal population genetic structure of C. andersoni in cattle, suggesting the absence of recombination among lineages. The finding of a clonal population genetic structure indicated that the prevalence of C. andersoni in cattle in Heilongjiang Province is not attributed to the introduction of cattle. Thus, prevention and control strategies should be focused on making stricter measures to avoid the occurrence of cross-transmission and re-infection between cattle individuals. These molecular data will also be helpful to explore the source attribution of infection/contamination of C. andersoni and to elucidate its transmission dynamics in Heilongjiang Province, even in China.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.