Evidence map›Paper›PMID 24999108›Full record

ArticleJournal of visualized experiments : JoVE2014

Dissection and downstream analysis of zebra finch embryos at early stages of development.

Jessica R Murray, Monika E Stanciauskas, Tejas S Aralere, Margaret S Saha

Open access · greenAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jessica R MurrayDepartment of Biology, College of William and Mary.
Monika E StanciauskasDepartment of Biology, College of William and Mary.
Tejas S AralereDepartment of Biology, College of William and Mary.
Margaret S SahaDepartment of Biology, College of William and Mary; mssaha@wm.edu.
William & Mary · US

Funding

Plasticity and Regulation in Xenopus Anterior-Posterior PatterningR15HD077624 · NICHD · COLLEGE OF WILLIAM AND MARY · PI SAHA, MARGARET S · 2013 to 2013
$368k
Neurotransmitter Fate Specification and the Role of Voltage-Gated Calcium ChannelR15NS067566 · NINDS · COLLEGE OF WILLIAM AND MARY · PI SAHA, MARGARET S · 2009 to 2009
$213k
Howard Hughes Medical InstituteNICHD NIH HHS R15 HD077624NINDS NIH HHS R15 NS067566NINDS NIH HHS R15NS067566
6 · The paper itself

Abstract

The zebra finch (Taeniopygiaguttata) has become an increasingly important model organism in many areas of research including toxicology, behavior, and memory and learning. As the only songbird with a sequenced genome, the zebra finch has great potential for use in developmental studies; however, the early stages of zebra finch development have not been well studied. Lack of research in zebra finch development can be attributed to the difficulty of dissecting the small egg and embryo. The following dissection method minimizes embryonic tissue damage, which allows for investigation of morphology and gene expression at all stages of embryonic development. This permits both bright field and fluorescence quality imaging of embryos, use in molecular procedures such as in situ hybridization (ISH), cell proliferation assays, and RNA extraction for quantitative assays such as quantitative real-time PCR (qtRT-PCR). This technique allows investigators to study early stages of development that were previously difficult to access.

Indexed as

AnimalsDissectionEmbryologyEmbryo, NonmammalianFemaleFinchesMaleModels, Animal

Identifiers

PMID24999108
PMCPMC4203306
OpenAlexW1992204055

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.