Evidence map›Paper›PMID 24998399›Full record

ArticleInsect biochemistry and molecular biology2014

Characterization of the Rel2-regulated transcriptome and proteome of Anopheles stephensi identifies new anti-Plasmodium factors.

Andrew Pike, Alekhya Vadlamani, Simone L Sandiford, Anthony Gacita, George Dimopoulos

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Evolution and assembly ofOpen biology · 2023
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  3. Inhibition ofPathogens (Basel, Switzerland) · 2020
    Article
  4. Article
  5. Article
  6. Article
  7. Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?International journal of environmental research and public health · 2017
    Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Andrew PikeW. Harry Feinstone Department of Molecular Microbiology and Immunology and the Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205-2179, USA. Electronic address: apike@jhsph.edu.
Alekhya VadlamaniW. Harry Feinstone Department of Molecular Microbiology and Immunology and the Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205-2179, USA. Electronic address: alvadlam@jhsph.edu.
Simone L SandifordW. Harry Feinstone Department of Molecular Microbiology and Immunology and the Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205-2179, USA. Electronic address: ssandifo@jhsph.edu.
Anthony GacitaW. Harry Feinstone Department of Molecular Microbiology and Immunology and the Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205-2179, USA. Electronic address: agacita1@jhu.edu.
George DimopoulosW. Harry Feinstone Department of Molecular Microbiology and Immunology and the Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205-2179, USA. Electronic address: gdimopou@jhsph.edu.
Johns Hopkins University · US

Funding

Training: Molecular & Cellular Bases of Infectious DiseasesT32AI007417 · NIAID · JOHNS HOPKINS UNIVERSITY · PI Arturo Casadevall · 1994 to 2026
$11.9M
Dissection of Anopheles Responses to Plasmodium Infected BloodR01AI061576 · NIAID · JOHNS HOPKINS UNIVERSITY · PI DIMOPOULOS, GEORGE · 2005 to 2014
$3.8M
Aedes anti-dengue immunityR01AI078997 · NIAID · JOHNS HOPKINS UNIVERSITY · PI DIMOPOULOS, GEORGE · 2009 to 2010
$805k
NIAID NIH HHS R01 AI061576NIAID NIH HHS R01AI061576NIAID NIH HHS R01 AI078997NIAID NIH HHS T32 AI007417
6 · The paper itself

Abstract

Mosquitoes possess an innate immune system that is capable of limiting infection by a variety of pathogens, including the Plasmodium spp. parasites responsible for human malaria. The Anopheles immune deficiency (IMD) innate immune signaling pathway confers resistance to Plasmodium falciparum. While some previously identified Anopheles anti-Plasmodium effectors are regulated through signaling by Rel2, the transcription factor of the IMD pathway, many components of this defense system remain uncharacterized. To begin to better understand the regulation of immune effector proteins by the IMD pathway, we used oligonucleotide microarrays and iTRAQ to analyze differences in mRNA and protein expression, respectively, between transgenic Anopheles stephensi mosquitoes exhibiting blood meal-inducible overexpression of an active recombinant Rel2 and their wild-type conspecifics. Numerous genes were differentially regulated at both the mRNA and protein levels following induction of Rel2. While multiple immune genes were up-regulated, a majority of the differentially expressed genes have no known immune function in mosquitoes. Selected up-regulated genes from multiple functional categories were tested for both anti-Plasmodium and anti-bacterial action using RNA interference (RNAi). Based on our experimental findings, we conclude that increased expression of the IMD immune pathway-controlled transcription factor Rel2 affects the expression of numerous genes with diverse functions, suggesting a broader physiological impact of immune activation and possible functional versatility of Rel2. Our study has also identified multiple novel genes implicated in anti-Plasmodium defense.

Indexed as

AnimalsAnimals, Genetically ModifiedAnophelesFemaleGene Expression ProfilingImmunity, InnateInsect ProteinsInsect VectorsOligonucleotide Array Sequence AnalysisPlasmodium falciparumProteomeRNA InterferenceSignal TransductionTranscription FactorsTranscriptomeUp-RegulationInsect ProteinsProteomeTranscription FactorsInnate immunityMosquitoesPlasmodiumProteomeRel2TranscriptomeTransgenesis

Identifiers

PMID24998399
PMCPMC4143444
OpenAlexW2014896057

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.