ArticleScientific reports2014
Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1).
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Whole exome sequencing as a screening tool in dogs: A pilot study.Computational and structural biotechnology journal · 2025Article
- Leading the pack: Best practices in comparative canine cancer genomics to inform human oncology.Veterinary and comparative oncology · 2023Review
- Article
- A domestic cat whole exome sequencing resource for trait discovery.Scientific reports · 2021Article
- A LINE-1 insertion situated in the promoter of IMPG2 is associated with autosomal recessive progressive retinal atrophy in Lhasa Apso dogs.BMC genetics · 2020Article
- Honey bee predisposition of resistance to ubiquitous mite infestations.Scientific reports · 2019Article
- Canine models of human amelogenesis imperfecta: identification of novel recessive ENAM and ACP4 variants.Human genetics · 2019Article
- Long-read sequence capture of the haemoglobin gene clusters across codfish species.Molecular ecology resources · 2019Article
- Whole exome sequencing in the rat.BMC genomics · 2018Article
- An exome sequencing based approach for genome-wide association studies in the dog.Scientific reports · 2017Article
- Exome sequencing reveals independent SGCD deletions causing limb girdle muscular dystrophy in Boston terriers.Skeletal muscle · 2017Article
- Identification of the PLA2G6 c.1579G>A Missense Mutation in Papillon Dog Neuroaxonal Dystrophy Using Whole Exome Sequencing Analysis.PloS one · 2017Article
- Exome sequencing reveals a nebulin nonsense mutation in a dog model of nemaline myopathy.Mammalian genome : official journal of the International Mammalian Genome Society · 2016Article
- Design and application of a target capture sequencing of exons and conserved non-coding sequences for the rat.BMC genomics · 2016Article
- Sequence capture by hybridization to explore modern and ancient genomic diversity in model and nonmodel organisms.Nucleic acids research · 2016Review
- An heuristic filtering tool to identify phenotype-associated genetic variants applied to human intellectual disability and canine coat colors.BMC bioinformatics · 2015Article
- Improved canine exome designs, featuring ncRNAs and increased coverage of protein coding genes.Scientific reports · 2015Article
- From caveman companion to medical innovator: genomic insights into the origin and evolution of domestic dogs.Advances in genomics and genetics · 2015Article
- Evaluation of whole-genome sequencing of four Chinese crested dogs for variant detection using the ion proton system.Canine genetics and epidemiology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.