ArticleScientific reports2014
WASH complex regulates Arp2/3 complex for actin-based polar body extrusion in mouse oocytes.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- The recurrent WASF1 nonsense variant identified in two unaffected Chinese families with neurodevelopmental disorder: case report and review of the literatures.BMC medical genomics · 2023Review
- Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes.Open biology · 2023Article
- A systems biology approach identifies the role of dysregulated PRDM6 in the development of hypertension.The Journal of clinical investigation · 2023Article
- Deletion of gmfA induces keratocyte-like migration in Dictyostelium.FEBS open bio · 2022Article
- Deliver on Time or Pay the Fine: Scheduling in Membrane Trafficking.International journal of molecular sciences · 2021Review
- Rac and Arp2/3-Nucleated Actin Networks Antagonize Rho During Mitotic and Meiotic Cleavages.Frontiers in cell and developmental biology · 2020Article
- Population and single‑cell transcriptome analyses reveal diverse transcriptional changes associated with radioresistance in esophageal squamous cell carcinoma.International journal of oncology · 2019Article
- Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development.Cell death & disease · 2018Article
- Quantitative proteomics of Sf21 cells during Baculovirus infection reveals progressive host proteome changes and its regulation by viral miRNA.Scientific reports · 2017Article
- Daam1 regulates fascin for actin assembly in mouse oocyte meiosis.Cell cycle (Georgetown, Tex.) · 2017Article
- Identification and functional analysis of differentially expressed genes in poorly differentiated hepatocellular carcinoma using RNA-seq.Oncotarget · 2017Article
- Genetic divergence in the transcriptional engram of chronic alcohol abuse: A laser-capture RNA-seq study of the mouse mesocorticolimbic system.Alcohol (Fayetteville, N.Y.) · 2017Article
- SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.Cell cycle (Georgetown, Tex.) · 2017Article
- Roles of actin binding proteins in mammalian oocyte maturation and beyond.Cell cycle (Georgetown, Tex.) · 2016Review
- Tropomodulin-3 is essential in asymmetric division during mouse oocyte maturation.Scientific reports · 2016Article
- The GTPase SPAG-1 orchestrates meiotic program by dictating meiotic resumption and cytoskeleton architecture in mouse oocytes.Molecular biology of the cell · 2016Article
- Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.Histochemistry and cell biology · 2016Article
- Cumulus Cell Transcripts Transit to the Bovine Oocyte in Preparation for Maturation.Biology of reproduction · 2016Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prior to their fertilization, oocytes undergo asymmetric division, which is regulated by actin filaments. Recently, WASH complex were identified as actin nucleation promoting factors (NPF) that activated Arp2/3 complex. However, the roles of WASH complex remain uncertain, particularly for oocyte polarization and asymmetric division. Here, we examined the functions of two important subunits of a WASH complex, WASH1 and Strumpellin, during mouse oocyte meiosis. Depleting WASH1 or disrupting Strumpellin activity by WASH1 morpholino (MO) injection or Strumpellin antibody injection decreased polar body extrusion and caused oocyte symmetric division, and this may have been due to spindle formation and migration defects. Time lapse microscopy showed that actin filaments distribution and relative amount at the membrane and in the cytoplasm of oocytes was significantly decreased after disrupting WASH complex. In addition, Arp2/3 complex expression was reduced after WASH1 depletion. Thus, our data indicated that WASH complex regulated Arp2/3 complex and were required for cytokinesis and following polar body extrusion during mouse oocyte meiotic maturation.
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