ArticleBiosensors & bioelectronics2014
Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.
Article in Biosensors & bioelectronics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Review
- A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nanopore Single-molecule Analysis of Biomarkers: Providing Possible Clues to Disease Diagnosis.Trends in analytical chemistry : TRAC · 2023Article
- A click chemistry amplified nanopore assay for ultrasensitive quantification of HIV-1 p24 antigen in clinical samples.Nature communications · 2022Article
- Article
- Nanopore detection of metal ions: Current status and future directions.Small methods · 2020Article
- Chemically functionalized conical PET nanopore for protein detection at the single-molecule level.Biosensors & bioelectronics · 2020Article
- Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors.Trends in biochemical sciences · 2020Review
- Single-molecule Study on the Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore.ACS applied bio materials · 2020Article
- A Nanopore Approach for Analysis of Caspase-7 Activity in Cell Lysates.Biophysical journal · 2019Article
- Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications.International journal of molecular sciences · 2019Review
- Salt-Mediated Nanopore Detection of ADAM-17.ACS applied bio materials · 2019Article
- Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices.Chemical communications (Cambridge, England) · 2018Article
- Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference.Biomaterials · 2018Article
- Graphene oxide-based biosensing platform for rapid and sensitive detection of HIV-1 protease.Analytical and bioanalytical chemistry · 2018Article
- Peptide-Mediated Nanopore Detection of Uranyl Ions in Aqueous Media.ACS sensors · 2017Article
- Nanopore Sensing.Analytical chemistry · 2017Review
- Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array.Nanoscale research letters · 2016Article
- Monitoring tetracycline through a solid-state nanopore sensor.Scientific reports · 2016Article
- Label-free nanopore single-molecule measurement of trypsin activity.ACS sensors · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10 min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.
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Registered trials
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