ArticleMolecules (Basel, Switzerland)2014
GSK3β regulates milk synthesis in and proliferation of dairy cow mammary epithelial cells via the mTOR/S6K1 signaling pathway.
Article in Molecules (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Epigenetic Effects and Potential Contributions of mAnimals : an open access journal from MDPI · 2025Article
- IL-13 May Could Enhance the Proliferation and Affect the Differentiation of Nasal Epithelium Basal Cells Through the mTOR/p70S6K1 Pathway in Chronic Rhinosinusitis With Nasal Polyps.Mediators of inflammation · 2025Article
- Dietary supplementation withFrontiers in veterinary science · 2024Article
- Article
- D-Methionine and 2-hydroxy-4-methylthiobutanoic acid i alter beta-casein, proteins and metabolites linked in milk protein synthesis in bovine mammary epithelial cells.Journal of animal science and technology · 2022Article
- Circadian GeneBiology · 2021Article
- UFL1 regulates milk protein and fat synthesis-related gene expression of bovine mammary epithelial cells probably via the mTOR signaling pathway.In vitro cellular & developmental biology. Animal · 2021Article
- Whole Genome Methylation Analysis Reveals Role of DNA Methylation in Cow's Ileal and Ileal Lymph Node Responses toFrontiers in genetics · 2021Article
- Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells.Asian-Australasian journal of animal sciences · 2020Article
- Camellia (Food science & nutrition · 2020Article
- mTORC2 Regulates Lipogenic Gene Expression through PPARBioMed research international · 2019Article
- BRD7 regulates the insulin-signaling pathway by increasing phosphorylation of GSK3β.Cellular and molecular life sciences : CMLS · 2018Article
- Article
- DEAD-box helicase 6 (DDX6) is a new negative regulator for milk synthesis and proliferation of bovine mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2018Article
- Phosphorylated mTOR Expression Profiles in Human Normal and Carcinoma Tissues.Disease markers · 2017Article
- Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells.Asian-Australasian journal of animal sciences · 2016Article
- Biological underpinnings of breastfeeding challenges: the role of genetics, diet, and environment on lactation physiology.American journal of physiology. Endocrinology and metabolism · 2016Review
- Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells.International journal of molecular sciences · 2015Article
- 14-3-3γ affects mTOR pathway and regulates lactogenesis in dairy cow mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2015Article
- Differential expression of GSK3β and pS9GSK3β in normal human tissues: can pS9GSK3β be an epithelial marker?International journal of clinical and experimental pathology · 2015Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase, whose activity is inhibited by AKT phosphorylation. This inhibitory phosphorylation of GSK3β can in turn play a regulatory role through phosphorylation of several proteins (such as mTOR, elF2B) to promote protein synthesis. mTOR is a key regulator in protein synthesis and cell proliferation, and recent studies have shown that both GSK3β and mTORC1 can regulate SREBP1 to promote fat synthesis. Thus far, however, the cross talk between GSK3β and the mTOR pathway in the regulation of milk synthesis and associated cell proliferation is not well understood. In this study the interrelationship between GSK3β and the mTOR/S6K1 signaling pathway leading to milk synthesis and proliferation of dairy cow mammary epithelial cells (DCMECs) was analyzed using techniques including GSK3β overexpression by transfection, GSK3β inhibition, mTOR inhibition and methionine stimulation. The analyses revealed that GSK3β represses the mTOR/S6K1 pathway leading to milk synthesis and cell proliferation of DCMECs, whereas GSK3β phosphorylation enhances this pathway. Conversely, the activated mTOR/S6K1 signaling pathway downregulates GSK3β expression but enhances GSK3β phosphorylation to increase milk synthesis and cell proliferation, whereas inhibition of mTOR leads to upregulation of GSK3β and repression of GSK3β phosphorylation, which in turn decreases milk synthesis, and cell proliferation. These findings indicate that GSK3β and phosphorylated GSK3β regulate milk synthesis and proliferation of DCMECs via the mTOR/S6K1 signaling pathway. These findings provide new insight into the mechanisms of milk synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.