ArticleTransfusion2014
Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay.
Article in Transfusion, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03056378 (Phase I Clinical Trial of Pooled Red Blood Cells for Transfusion), which is not on this map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Phase I Clinical Trial of Pooled Red Blood Cells for Transfusion
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Global meta-analysis onPathogens and global health · 2022Pooled it
- Guidance on the Management of Asymptomatic Blood Donors Who Test Positive for Babesia.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- Babesiosis and Its Significance in Transfusion Medicine from a European Point of View.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2026Review
- A Highly Sensitive Multiplex Antibody Assay Reduces Window Period for Detection ofOpen forum infectious diseases · 2025Article
- Transfusion-transmittedJournal of clinical microbiology · 2023Review
- Leveraging Donor Populations to Study the Epidemiology and Pathogenesis of Transfusion-Transmitted and Emerging Infectious Diseases.Transfusion medicine reviews · 2023Article
- Identification and characterization of extracellular vesicles from red cells infected withFrontiers in cellular and infection microbiology · 2022Article
- Preventing Transfusion-Transmitted Babesiosis.Pathogens (Basel, Switzerland) · 2021Review
- The Babesia observational antibody (BAOBAB) study: A cross-sectional evaluation of Babesia in two communities in Kilosa district, Tanzania.PLoS neglected tropical diseases · 2019Article
- Persistence ofPathogens (Basel, Switzerland) · 2019Review
- A library of recombinant Babesia microti cell surface and secreted proteins for diagnostics discovery and reverse vaccinology.International journal for parasitology · 2019Article
- Performance Evaluation of a Prototype Architect Antibody Assay for Babesia microti.Journal of clinical microbiology · 2018Article
- Transfusion-transmitted babesiosis: is it time to screen the blood supply?Current opinion in hematology · 2016Review
- Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions.Scientific reports · 2016Article
- A targeted immunomic approach identifies diagnostic antigens in the human pathogen Babesia microti.Transfusion · 2016Article
- Serologic screening of United States blood donors for Babesia microti using an investigational enzyme immunoassay.Transfusion · 2016Article
- A prospective evaluation of chronic Babesia microti infection in seroreactive blood donors.Transfusion · 2016Article
- Experimental transfusion-induced Babesia microti infection: dynamics of parasitemia and immune responses in a rhesus macaque model.Transfusion · 2016Article
- Review
- Risks associated with red blood cell transfusions: potential benefits from application of pathogen inactivation.Transfusion · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 7 institutions in 1 country.
Funding
Abstract
backgroundTransfusion-transmitted babesiosis caused by Babesia microti has emerged as a significant risk to the US blood supply. This study estimated the prevalence of B. microti antibodies in blood donors using an investigational enzyme immunoassay (EIA). STUDY DESIGN AND
methodsA peptide-based EIA that detects both immunoglobulin (Ig)G and IgM antibodies to B. microti was developed and validated. Donor samples randomly selected from areas defined as high-risk endemic, lower-risk endemic, and nonendemic for B. microti were deidentified and tested using the investigational EIA. Samples that were EIA repeat reactive were further tested by B. microti immunofluorescent assay (IFA), polymerase chain reaction (PCR) on red blood cell lysates, and peripheral blood smear examination. A random subset of 1272 samples from high-risk endemic areas was tested by IFA, PCR, and peripheral blood smear in parallel with EIA.
resultsAmong 15,000 donations tested with the investigational B. microti EIA, EIA repeat-reactive rates were 1.08% (54/5000) in a high-risk endemic area, 0.74% (37/5000) in a lower-risk area, and 0.40% (20/5000) in a nonendemic area. After application of a revised cutoff, these values were reduced to 0.92%, (46/5000), 0.54% (27/5000), and 0.16% (8/5000). Overall concordance between EIA and IFA among donor samples was 99.34%. One seropositive sample was positive by PCR.
conclusionThe seroprevalence of B. microti in blood donors in a high-risk area measured by an investigational EIA was approximately 1%. The EIA shows promise as an efficient high-throughput blood donor screening assay for B. microti.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.