ArticleMolecular microbiology2014
Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Multitier regulation of theJournal of bacteriology · 2024Article
- EvgS/EvgA, the unorthodox two-component system regulating bacterial multiple resistance.Applied and environmental microbiology · 2023Review
- Creating Polyploid Escherichia Coli and Its Application in Efficient L-Threonine Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Enterococcus faecalis OG1RF Evolution at Low pH Selects Fusidate-Sensitive Mutants in Elongation Factor G and at High pH Selects Defects in Phosphate Transport.Applied and environmental microbiology · 2023Article
- Heterogeneity and Evolutionary Tunability of Escherichia coli Resistance against Extreme Acid Stress.Microbiology spectrum · 2022Article
- Roles of Two-Component Signal Transduction Systems in Shigella Virulence.Biomolecules · 2022Review
- The challenges and prospects of Escherichia coli as an organic acid production host under acid stress.Applied microbiology and biotechnology · 2021Review
- Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.Biomolecules · 2021Review
- Extreme Acid Modulates Fitness Trade-Offs of Multidrug Efflux Pumps MdtEF-TolC and AcrAB-TolC in Escherichia coli K-12.Applied and environmental microbiology · 2021Article
- Structural insight into the role of the PAS domainfor signal transduction in sensor-kinase BvgS.Journal of bacteriology · 2021Article
- Gain-of-Function Mutations in Acid Stress Response (Antimicrobial agents and chemotherapy · 2021Article
- Biocide-Induced Emergence of Antibiotic Resistance inFrontiers in microbiology · 2021Article
- Acid-Sensing Histidine Kinase With a Redox Switch.Frontiers in microbiology · 2021Article
- Comparative Review of the Responses ofGenes · 2020Review
- The Signaling Molecule Indole Inhibits Induction of the AR2 Acid Resistance System inFrontiers in microbiology · 2020Article
- Inverted Regulation of Multidrug Efflux Pumps, Acid Resistance, and Porins in Benzoate-Evolved Escherichia coli K-12.Applied and environmental microbiology · 2019Article
- Natural variation of a sensor kinase controlling a conserved stress response pathway in Escherichia coli.PLoS genetics · 2017Article
- Structural and Functional Analysis of the Escherichia coli Acid-Sensing Histidine Kinase EvgS.Journal of bacteriology · 2017Article
- Benzoate- and Salicylate-Tolerant Strains of Escherichia coli K-12 Lose Antibiotic Resistance during Laboratory Evolution.Applied and environmental microbiology · 2017Article
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
Laboratory-based evolution and whole-genome sequencing can link genotype and phenotype. We used evolution of acid resistance in exponential phase Escherichia coli to study resistance to a lethal stress. Iterative selection at pH 2.5 generated five populations that were resistant to low pH in early exponential phase. Genome sequencing revealed multiple mutations, but the only gene mutated in all strains was evgS, part of a two-component system that has already been implicated in acid resistance. All these mutations were in the cytoplasmic PAS domain of EvgS, and were shown to be solely responsible for the resistant phenotype, causing strong upregulation at neutral pH of genes normally induced by low pH. Resistance to pH 2.5 in these strains did not require the transporter GadC, or the sigma factor RpoS. We found that EvgS-dependent constitutive acid resistance to pH 2.5 was retained in the absence of the regulators GadE or YdeO, but was lost if the oxidoreductase YdeP was also absent. A deletion in the periplasmic domain of EvgS abolished the response to low pH, but not the activity of the constitutive mutants. On the basis of these results we propose a model for how EvgS may become activated by low pH.
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