ArticleMolecular microbiology2014
De novo morphogenesis in L-forms via geometric control of cell growth.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 77 citations in OpenAlex.
- Bacterial growth under confinement requires transcriptional adaptation to resist turgor pressure build-up.Nature communications · 2026Article
- Sensing the shape of a surface by tightly surface-bound filaments.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Septal wall synthesis is sufficient to change ameba-like cells into uniform oval-shaped cells in Escherichia coli L-forms.Communications biology · 2024Article
- Escherichia coli has robust regulatory mechanisms against elevated peptidoglycan cleavage by lytic transglycosylases.The Journal of biological chemistry · 2023Article
- Recovery of Vibrio cholerae polarized cellular organization after exit from a non-proliferating spheroplast state.PloS one · 2023Article
- Enzyme 1 of the phosphoenolpyruvate:sugar phosphotransferase system is involved in resistance to MreB disruption in wild-type and ∆envC cells.Molecular microbiology · 2022Article
- Effects of antibiotics on bacterial cell morphology and their physiological origins.Biochemical Society transactions · 2022Review
- Physical properties of the bacterial outer membrane.Nature reviews. Microbiology · 2022Review
- High-level carbapenem tolerance requires antibiotic-induced outer membrane modifications.PLoS pathogens · 2022Article
- Refractory Osteomyelitis Caused byInfection and drug resistance · 2022Article
- The wall-less bacteriumiScience · 2021Article
- Review
- Precise regulation of the relative rates of surface area and volume synthesis in bacterial cells growing in dynamic environments.Nature communications · 2021Article
- Direct Observation of Conversion From Walled Cells to Wall-Deficient L-Form andFrontiers in microbiology · 2021Article
- Article
- Establishing rod shape from spherical, peptidoglycan-deficient bacterial spores.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Spheroplast-Mediated Carbapenem Tolerance in Gram-Negative Pathogens.Antimicrobial agents and chemotherapy · 2019Article
- Mechanics and Dynamics of Bacterial Cell Lysis.Biophysical journal · 2019Article
- DivIVA concentrates mycobacterial cell envelope assembly for initiation and stabilization of polar growth.Cytoskeleton (Hoboken, N.J.) · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In virtually all bacteria, the cell wall is crucial for mechanical integrity and for determining cell shape. Escherichia coli's rod-like shape is maintained via the spatiotemporal patterning of cell-wall synthesis by the actin homologue MreB. Here, we transiently inhibited cell-wall synthesis in E. coli to generate cell-wall-deficient, spherical L-forms, and found that they robustly reverted to a rod-like shape within several generations after inhibition cessation. The chemical composition of the cell wall remained essentially unchanged during this process, as indicated by liquid chromatography. Throughout reversion, MreB localized to inwardly curved regions of the cell, and fluorescent cell wall labelling revealed that MreB targets synthesis to those regions. When exposed to the MreB inhibitor A22, reverting cells regrew a cell wall but failed to recover a rod-like shape. Our results suggest that MreB provides the geometric measure that allows E. coli to actively establish and regulate its morphology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.