ArticleJournal of applied physiology (Bethesda, Md. : 1985)2014
Intrinsic stiffness of extracellular matrix increases with age in skeletal muscles of mice.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
125 citing papers in PubMed, 3 syntheses or guidelines pooled it, 209 citations in OpenAlex.
- Muscle elasticity variations in assessing age-related changes in adults - A systematic review.Geriatrics & gerontology international · 2025Pooled it
- Exploring the associations between skeletal muscle echogenicity and physical function in aging adults: A systematic review with meta-analyses.Journal of sport and health science · 2024Pooled it
- IsGenes · 2022Pooled it
- The nucleus as a mechanobiological hub in muscle aging.Nucleus (Austin, Tex.) · 2026Review
- The senescence-stiffening loop: Extracellular matrix remodeling, hypoperfusion, and mitochondrial dysfunction drive tissue aging.Cell metabolism · 2026Review
- Macrophage regulation of extracellular matrix remodeling in aging skeletal muscle.Ageing research reviews · 2026Review
- Integrated Opto-Biomechatronics For Single Muscle Fibre Structure-Function Assessment: The MyoRobot 3.0.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Non-linear Characterization of Commercial and Decellularized Hydrogels: Statistical Framework Enhanced by Bayesian Optimization.Cellular and molecular bioengineering · 2026Article
- Sex-specific effects of fatiguing exercise on skeletal muscle passive mechanics are preserved in aging.bioRxiv : the preprint server for biology · 2026Article
- Quantification of muscle morphology and stiffness in spastic hemiplegic cerebral palsy versus typically developing controls using magnetic resonance elastography.Clinical biomechanics (Bristol, Avon) · 2026Article
- Mechanobiological landscape of muscle stem cells.Skeletal muscle · 2026Review
- How muscle ageing affects rapid goal-directed movement: mechanistic insights from a simple model.PLoS computational biology · 2026Article
- Piezo1-dependent activation of stromal cells ignites muscle inflammation in exercise and injury and is associated with inflammaging.Nature immunology · 2026Article
- Overactive PDGFRα and PDGFRβ promote distinct yet overlapping phenotypes of skeletal muscle fibrosis and stiffness, with PDGFRβ also driving drastic muscle growth.bioRxiv : the preprint server for biology · 2026Article
- Age-related changes in quadriceps stiffness and strength: a longitudinal observation in community-dwelling older adults.BMC geriatrics · 2025Observational
- Aging in the Skeletal Muscle Revealed by Molecular Immunohistochemical Imaging.International journal of molecular sciences · 2025Review
- Quercetin, Kaempferol and Capsaicin Counteract the TGF-β1-Induced Upregulation of αSMA and Collagen in Myoblasts.International journal of molecular sciences · 2025Article
- A cross-sectional study on peroneal muscle echogenicity changes and their effects on balance functions in individuals with chronic ankle instability.Scientific reports · 2025Article
- Spiny mice (Acomys) have evolved cellular features to support regenerative healing.Annals of the New York Academy of Sciences · 2025Review
- Aging-associated differences between perioral and trunk muscle characteristics.Scientific reports · 2024Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Advanced age is associated with increases in muscle passive stiffness, but the contributors to the changes remain unclear. Our purpose was to determine the relative contributions of muscle fibers and extracellular matrix (ECM) to muscle passive stiffness in both adult and old animals. Passive mechanical properties were determined for isolated individual muscle fibers and bundles of muscle fibers that included their associated ECM, obtained from tibialis anterior muscles of adult (8-12 mo old) and old (28-30 mo old) mice. Maximum tangent moduli of individual muscle fibers from adult and old muscles were not different at any sarcomere length tested. In contrast, the moduli of bundles of fibers from old mice was more than twofold greater than that of fiber bundles from adult muscles at sarcomere lengths >2.5 μm. Because ECM mechanical behavior is determined by the composition and arrangement of its molecular constituents, we also examined the effect of aging on ECM collagen characteristics. With aging, muscle ECM hydroxyproline content increased twofold and advanced glycation end-product protein adducts increased threefold, whereas collagen fibril orientation and total ECM area were not different between muscles from adult and old mice. Taken together, these findings indicate that the ECM of tibialis anterior muscles from old mice has a higher modulus than the ECM of adult muscles, likely driven by an accumulation of densely packed extensively crosslinked collagen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.