ArticleParasites & vectors2014
Phylogenetic study of six species of Anopheles mosquitoes in Peninsular Malaysia based on inter-transcribed spacer region 2 (ITS2) of ribosomal DNA.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A review and updated checklist of the Anopheles mosquitoes (Diptera: Culicidae: Anophelinae) of Thailand.Journal of medical entomology · 2026Review
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- Diversity of Anopheles species and zoonotic malaria vector of the Buton Utara Wildlife Sanctuary, Southeast Sulawesi, Indonesia.Malaria journal · 2023Article
- High transmission efficiency of the simian malaria vectors and population expansion of their parasites Plasmodium cynomolgi and Plasmodium inui.PLoS neglected tropical diseases · 2023Article
- Genetic diversity, phylogenetic and phylogeographic analysis of Anopheles culicifacies species complex using ITS2 and COI sequences.PloS one · 2023Article
- Morphological and molecular identification reveals a high diversity of Anopheles species in the forest region of the Cambodia-Laos border.Parasites & vectors · 2022Article
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- Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies.Scientific reports · 2022Article
- Redesigning Primer of ITS2 (Internal Transcribed Spacer 2) for Specific Molecular Characterization of Malaria Vectors Anopheles Species.Medical archives (Sarajevo, Bosnia and Herzegovina) · 2021Article
- Evaluation of Mosquito Magnet and other collection tools for Anopheles mosquito vectors of simian malaria.Parasites & vectors · 2021Article
- New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi.Parasites & vectors · 2020Article
- A Multiplex PCR Based on MitochondrialInsects · 2020Article
- Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2.G3 (Bethesda, Md.) · 2017Article
- Composition and Genetic Diversity of Mosquitoes (Diptera: Culicidae) on Islands and Mainland Shores of Kenya's Lakes Victoria and Baringo.Journal of medical entomology · 2016Article
- Article
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8 authors.
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Abstract
backgroundMolecular techniques are invaluable for investigation on the biodiversity of Anopheles mosquitoes. This study aimed at investigating the spatial-genetic variations among Anopheles mosquitoes from different areas of Peninsular Malaysia, as well as deciphering evolutionary relationships of the local Anopheles mosquitoes with the mosquitoes from neighbouring countries using the anopheline ITS2 rDNA gene.
methodsMosquitoes were collected, identified, dissected to check infection status, and DNA extraction was performed for PCR with primers targeting the ITS2 rDNA region. Sequencing was done and phylogenetic tree was constructed to study the evolutionary relationship among Anopheles mosquitoes within Peninsular Malaysia, as well as across the Asian region.
resultsA total of 133 Anopheles mosquitoes consisting of six different species were collected from eight different locations across Peninsular Malaysia. Of these, 65 ITS2 rDNA sequences were obtained. The ITS2 rDNA amplicons of the studied species were of different sizes. One collected species, Anopheles sinensis, shows two distinct pools of population in Peninsular Malaysia, suggesting evolvement of geographic race or allopatric speciation.
conclusionAnopheles mosquitoes from Peninsular Malaysia show close evolutionary relationship with the Asian anophelines. Nevertheless, genetic differences due to geographical segregation can be seen. Meanwhile, some Anopheles mosquitoes in Peninsular Malaysia show vicariance, exemplified by the emergence of distinct cluster of An. sinensis population.
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