ArticleAnalytical chemistry2014
3D-printed microfluidic microdissector for high-throughput studies of cellular aging.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Nonlinear memory in cell-division dynamics across species.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Features of Vat-Photopolymerized Masters for Microfluidic Device Manufacturing.Bioengineering (Basel, Switzerland) · 2024Article
- High-Efficiency Single-Cell Containment Microdevices Based on Fluid Control.Micromachines · 2023Article
- Cancer Stem Cells in Tumor Modeling: Challenges and Future Directions.Advanced nanobiomed research · 2021Article
- Multi-Organs-on-Chips for Testing Small-Molecule Drugs: Challenges and Perspectives.Pharmaceutics · 2021Review
- Patient-Specific Organoid and Organ-on-a-Chip: 3D Cell-Culture Meets 3D Printing and Numerical Simulation.Advanced biology · 2021Review
- Isolating live cells after high-throughput, long-term, time-lapse microscopy.Nature methods · 2020Article
- 3D-printed miniaturized fluidic tools in chemistry and biology.Trends in analytical chemistry : TRAC · 2018Article
- Article
- Microfluidics and single-cell microscopy to study stochastic processes in bacteria.Current opinion in microbiology · 2018Review
- Emerging Anti-Fouling Methods: Towards Reusability of 3D-Printed Devices for Biomedical Applications.Micromachines · 2018Article
- A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells.Bio-protocol · 2018Article
- 3D-glass molds for facile production of complex droplet microfluidic chips.Biomicrofluidics · 2018Article
- 3D-Printing of Functional Biomedical Microdevices via Light- and Extrusion-Based Approaches.Small methods · 2018Article
- Article
- Article
- 3D-printed bioanalytical devices.Nanotechnology · 2016Review
- Single-Cell Physiology.Annual review of biophysics · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Due to their short lifespan, rapid division, and ease of genetic manipulation, yeasts are popular model organisms for studying aging in actively dividing cells. To study replicative aging over many cell divisions, individual cells must be continuously separated from their progeny via a laborious manual microdissection procedure. Microfluidics-based soft-lithography devices have recently been used to automate microdissection of the budding yeast Saccharomyces cerevisiae. However, little is known about replicative aging in Schizosaccharomyces pombe, a rod-shaped yeast that divides by binary fission and shares many conserved biological functions with higher eukaryotes. In this report, we develop a versatile multiphoton lithography method that enables rapid fabrication of three-dimensional master structures for polydimethylsiloxane (PDMS)-based microfluidics. We exploit the rapid prototyping capabilities of multiphoton lithography to create and characterize a cell-capture device that is capable of high-resolution microscopic observation of hundreds of individual S. pombe cells. By continuously removing the progeny cells, we demonstrate that cell growth and protein aggregation can be tracked in individual cells for over ~100 h. Thus, the fission yeast lifespan microdissector (FYLM) provides a powerful on-chip microdissection platform that will enable high-throughput studies of aging in rod-shaped cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.