ArticleCancer gene therapy2014
Electroporation markedly improves Sleeping Beauty transposon-induced tumorigenesis in mice.
Article in Cancer gene therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Optimization of piggyBac Transposon System Electrotransfection in Sheep Fibroblasts.Molecular biotechnology · 2023Article
- Gene delivery in adherent and suspension cells using the combined physical methods.Cytotechnology · 2022Article
- Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts.BMC molecular and cell biology · 2020Article
- Cancer modeling by Transgene Electroporation in Adult Zebrafish (TEAZ).Disease models & mechanisms · 2018Article
- Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.Nature communications · 2018Article
- Development of a Mouse Model of Prostate Cancer Using the Sleeping Beauty Transposon and Electroporation.Molecules (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Sleeping Beauty (SB) transposon system is an important tool for genetic studies. It is used to insert a gene of interest into the host chromosome, thus enabling permanent gene expression. However, this system is less useful in higher eukaryotes because the transposition frequency is low. Efforts to improve the efficacy of the SB transposon system have focused on the method of gene delivery, but although electroporation has recently attracted much attention as an in vivo gene delivery tool, the simultaneous use of electroporation and the SB transposon system has not been studied for gene transfer in mice. In this study, electroporation was used in a model of SB transposon-induced insertional tumorigenesis. Electroporation increased the rate of tumor development to three times that of the control group. There was no difference in phenotype between tumors induced with the SB transposon system alone and those induced by the SB transposon and electroporation. Electroporation therefore may be an efficient means of improving the efficacy of gene transfer via the SB transposon system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.