ArticleMetallomics : integrated biometal science2014
3D imaging of transition metals in the zebrafish embryo by X-ray fluorescence microtomography.
Article in Metallomics : integrated biometal science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Preservation artifacts in zebrafish X-ray fluorescence imaging.Metallomics : integrated biometal science · 2026Article
- Unimodal Imaging of Monovalent Metal-Chelator Complexes and Lipids by MALDI Imaging Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2025Article
- Biotransformation and biological fate of magnetic iron oxide nanoparticles for biomedical research and clinical applications.Nanoscale advances · 2025Review
- Metallomic mapping of gut and brain in heavy metal exposed earthworms: A novel paradigm in ecotoxicology.Biochemical and biophysical research communications · 2024Article
- A High-Sensitivity Benchtop X-Ray Fluorescence Emission Tomography (XFET) System With a Full-Ring of X-Ray Imaging-Spectrometers and a Compound-Eye Collimation Aperture.IEEE transactions on medical imaging · 2024Article
- Zebrafish (Danio rerio) larvae as a predictive model to study gentamicin-induced structural alterations of the kidney.PloS one · 2023Article
- Development of Multi-Scale X-ray Fluorescence Tomography for Examination of Nanocomposite-Treated Biological Samples.Cancers · 2021Article
- The ZIP6/ZIP10 heteromer is essential for the zinc-mediated trigger of mitosis.Cellular and molecular life sciences : CMLS · 2021Article
- Use of X-Ray Fluorescence Microscopy for Studies on Research Models of Hepatocellular Carcinoma.Frontiers in public health · 2021Article
- Optical Control of Metal Ion Probes in Cells and Zebrafish Using Highly Selective DNAzymes Conjugated to Upconversion Nanoparticles.Journal of the American Chemical Society · 2018Article
- Biogenesis of zinc storage granules inThe Journal of experimental biology · 2018Article
- 3D chemical imaging of the brain using quantitative IR spectro-microscopy.Chemical science · 2018Article
- Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling.Analytical chemistry · 2017Review
- Article
- In vivo bioluminescence imaging reveals copper deficiency in a murine model of nonalcoholic fatty liver disease.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- 3D Imaging of Nanoparticle Distribution in Biological Tissue by Laser-Induced Breakdown Spectroscopy.Scientific reports · 2016Article
- In Situ Microfocus Chemical Computed Tomography of the Composition of a Single Catalyst Particle During Hydrogenation of Nitrobenzene in the Liquid Phase.Angewandte Chemie (International ed. in English) · 2015Article
- Synthetic fluorescent probes for studying copper in biological systems.Chemical Society reviews · 2015Review
- A high-throughput system for high-quality tomographic reconstruction of large datasets at Diamond Light Source.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Synchrotron X-ray fluorescence (SXRF) microtomography has emerged as a powerful technique for the 3D visualization of the elemental distribution in biological samples. The mechanical stability, both of the instrument and the specimen, is paramount when acquiring tomographic projection series. By combining the progressive lowering of temperature method (PLT) with femtosecond laser sectioning, we were able to embed, excise, and preserve a zebrafish embryo at 24 hours post fertilization in an X-ray compatible, transparent resin for tomographic elemental imaging. Based on a data set comprised of 60 projections, acquired with a step size of 2 μm during 100 hours of beam time, we reconstructed the 3D distribution of zinc, iron, and copper using the iterative maximum likelihood expectation maximization (MLEM) reconstruction algorithm. The volumetric elemental maps, which entail over 124 million individual voxels for each transition metal, revealed distinct elemental distributions that could be correlated with characteristic anatomical features at this stage of embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.