ArticlePLoS pathogens2014
Characterization of the largest effector gene cluster of Ustilago maydis.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 111 citations in OpenAlex.
- Ustilago maydis Phosphatidic Acid and Phosphatidylserine-Targeting Secreted Lipase Lip3 Is Involved in Pathogenesis.Molecular plant pathology · 2026Article
- Pathogenic fungus Ustilago maydis exploits the lateral root regulators to induce pluripotency in maize shoots.The New phytologist · 2026Article
- A Genomic DNA-Based Real-Time PCR Assay for Quantification of Ustilago maydis Biomass in Plant Samples.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Jasmonic acid signalling is targeted by a smut fungal Tin2-fold effector.Journal of experimental botany · 2025Article
- Surface frustration re-patterning underlies the structural landscape and evolvability of fungal orphan candidate effectors.Nature communications · 2023Article
- The nuclear effector MoHTR3 of Magnaporthe oryzae modulates host defence signalling in the biotrophic stage of rice infection.Molecular plant pathology · 2023Article
- Progress in pathogenesis research of Ustilago maydis, and the metabolites involved along with their biosynthesis.Molecular plant pathology · 2023Review
- Functional Clustering of Metabolically Related Genes Is Conserved acrossJournal of fungi (Basel, Switzerland) · 2023Review
- Article
- Virulence and pathogenicity determinants in whole genome sequence ofFrontiers in microbiology · 2023Article
- Systematic characterization ofFrontiers in microbiology · 2023Article
- Prediction of effector protein structures from fungal phytopathogens enables evolutionary analyses.Nature microbiology · 2023Article
- An Endoglucanase Secreted byJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- TheJournal of fungi (Basel, Switzerland) · 2022Article
- A sugarcane smut fungus effector simulates the host endogenous elicitor peptide to suppress plant immunity.The New phytologist · 2022Article
- The small-secreted cysteine-rich protein CyrA is a virulence factor participating in the attack of Caenorhabditis elegans by Duddingtonia flagrans.PLoS pathogens · 2021Article
- Pathogens pulling the strings: Effectors manipulating salicylic acid and phenylpropanoid biosynthesis in plants.Molecular plant pathology · 2021Review
- Population Genomics of the Maize Pathogen Ustilago maydis: Demographic History and Role of Virulence Clusters in Adaptation.Genome biology and evolution · 2021Article
- A fungal member of theeLife · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the genome of the biotrophic plant pathogen Ustilago maydis, many of the genes coding for secreted protein effectors modulating virulence are arranged in gene clusters. The vast majority of these genes encode novel proteins whose expression is coupled to plant colonization. The largest of these gene clusters, cluster 19A, encodes 24 secreted effectors. Deletion of the entire cluster results in severe attenuation of virulence. Here we present the functional analysis of this genomic region. We show that a 19A deletion mutant behaves like an endophyte, i.e. is still able to colonize plants and complete the infection cycle. However, tumors, the most conspicuous symptoms of maize smut disease, are only rarely formed and fungal biomass in infected tissue is significantly reduced. The generation and analysis of strains carrying sub-deletions identified several genes significantly contributing to tumor formation after seedling infection. Another of the effectors could be linked specifically to anthocyanin induction in the infected tissue. As the individual contributions of these genes to tumor formation were small, we studied the response of maize plants to the whole cluster mutant as well as to several individual mutants by array analysis. This revealed distinct plant responses, demonstrating that the respective effectors have discrete plant targets. We propose that the analysis of plant responses to effector mutant strains that lack a strong virulence phenotype may be a general way to visualize differences in effector function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.