ArticleNature protocols2014
Flux profiling of photosynthetic carbon metabolism in intact plants.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- Age-based approach to characterize the dynamics of cellular processes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Variations in carbon flux allocation among cassava (Manihot esculenta) cultivars arise from balanced competition between starch accumulation and structural component development.Communications biology · 2026Article
- Stable Isotope Labeling and Quantification of Photosynthetic Metabolites.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Evolution of gene regulatory network of CPlant communications · 2023Article
- An Arabidopsis GCMS chemical ionization technique to quantify adaptive responses in central metabolism.Plant physiology · 2022Article
- Rising rates of starch degradation during daytime and trehalose 6-phosphate optimize carbon availability.Plant physiology · 2022Article
- An integrated isotopic labeling and freeze sampling apparatus (ILSA) to support sampling leaf metabolomics at a centi-second scale.Plant methods · 2022Article
- Recent advances in proteomics and metabolomics in plants.Molecular horticulture · 2022Review
- Point mutations that boost aromatic amino acid production and COScience advances · 2022Article
- Design and fabrication of an improved dynamic flow cuvette forPlant methods · 2022Article
- Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, CNature plants · 2022Article
- Review
- Photorespiration: The Futile Cycle?Plants (Basel, Switzerland) · 2021Review
- The Contribution of Metabolomics to Systems Biology: Current Applications Bridging Genotype and Phenotype in Plant Science.Advances in experimental medicine and biology · 2021Article
- Recent Advances in Carbon and Nitrogen Metabolism in C3 Plants.International journal of molecular sciences · 2020Review
- Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom.Nature communications · 2019Article
- Advances in metabolic flux analysis toward genome-scale profiling of higher organisms.Bioscience reports · 2018Review
- Metabolite pools and carbon flow during C4 photosynthesis in maize: 13CO2 labeling kinetics and cell type fractionation.Journal of experimental botany · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flux analysis has been carried out in plants for decades, but technical innovations are now enabling it to be carried out in photosynthetic tissues in a more precise fashion with respect to the number of metabolites measured. Here we describe a protocol, using gas chromatography (GC)- and liquid chromatography (LC)-mass spectrometry (MS), to resolve intracellular fluxes of the central carbon metabolism in illuminated intact Arabidopsis thaliana rosettes using the time course of the unlabeled fractions in 40 major constituents of the metabolome after switching to (13)CO2. We additionally simplify modeling assumptions, specifically to cope with the presence of multiple cellular compartments. We summarize all steps in this 8-10-week-long process, including setting up the chamber; harvesting; liquid extraction and subsequent handling of sample plant material to chemical derivatization procedures such as silylation and methoxymation (necessary for gas chromatography only); choosing instrumentation settings and evaluating the resultant chromatogram in terms of both unlabeled and labeled peaks. Furthermore, we describe how quantitative insights can be gained by estimating both benchmark and previously unknown fluxes from collected data sets.
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