ArticleExperimental cell research2014
The formation of cortical actin arrays in human trabecular meshwork cells in response to cytoskeletal disruption.
Article in Experimental cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Cross-linked actin networks (CLANs) affect stiffness and/or actin dynamics in transgenic transformed and primary human trabecular meshwork cells.Experimental eye research · 2022Article
- The Dual Effect of Rho-Kinase Inhibition on Trabecular Meshwork Cells Cytoskeleton and Extracellular Matrix in an In Vitro Model of Glaucoma.Journal of clinical medicine · 2022Article
- Ripasudil in a Model of Pigmentary Glaucoma.Translational vision science & technology · 2020Article
- Impact of pigment dispersion on trabecular meshwork cells.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Establishment of human trabecular meshwork cell cultures using nontransplantable corneoscleral rims.Turkish journal of biology = Turk biyoloji dergisi · 2019Article
- Cross-linked actin networks (CLANs) in glaucoma.Experimental eye research · 2017Review
- RhoA/ROCK signaling regulates TGFβ-induced epithelial-mesenchymal transition of lens epithelial cells through MRTF-A.Molecular medicine (Cambridge, Mass.) · 2016Article
- The intrinsic stiffness of human trabecular meshwork cells increases with senescence.Oncotarget · 2015Article
- Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells.Experimental eye research · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The cytoskeleton of human trabecular meshwork (HTM) cells is known to be altered in glaucoma and has been hypothesized to reduce outflow facility through contracting the HTM tissue. Latrunculin B (Lat-B) and Rho-associated protein kinase (ROCK) inhibitors disrupt the actin cytoskeleton and are in clinical trials as glaucoma therapeutics. We have previously reported a transient increase in HTM cell stiffness peaking at 90 min after Lat-B treatment with a return to pretreatment values after 270 min. We hypothesize that changes in actin morphology correlate with alterations in cell stiffness induced by Lat-B but this is not a general consequence of other cytoskeletal disrupting agents such as Rho kinase inhibitors. We treated HTM cells with 2 µM Lat-B or 100 µM Y-27632 and allowed the cells to recover for 30-270 min. While examining actin morphology in Lat-B treated cells, we observed striking cortical actin arrays (CAAs). The percentage of CAA positive cells (CPCs) was time dependent and exceeded 30% at 90 min and decreased after 270 min. Y-27632 treated cells exhibited few CAAs and no changes in cell stiffness. Together, these data suggest that the increase in cell stiffness after Lat-B treatment is correlated with CAAs.
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