ArticleMolecular vision2014
Interleukin-4 and melatonin ameliorate high glucose and interleukin-1β stimulated inflammatory reaction in human retinal endothelial cells and retinal pigment epithelial cells.
Article in Molecular vision, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Retinal pigment epithelium pathology in age-related macular degeneration: mitigation with melatonin.Frontiers in medicine · 2026Review
- Biochemical Study of Bilberry Extract Potential in Preventing Retinal Damage in Rat Model of Diabetes Induced by Streptozotocin/Nicotinamide.Life (Basel, Switzerland) · 2025Article
- Evaluation of the effect of melatonin treatment on telomere length of the retinal pigment epithelium in streptozotocin-induced diabetic rat model.BMC ophthalmology · 2024Article
- The Role and Therapeutic Potential of Melatonin in Degenerative Fundus Diseases: Diabetes Retinopathy and Age-Related Macular Degeneration.Drug design, development and therapy · 2024Review
- Substance P Alleviates Retinal Pigment Epithelium Dysfunction Caused by High Glucose-Induced Stress.Life (Basel, Switzerland) · 2023Article
- Inflammatory cytokines as mediators of retinal endothelial barrier dysfunction in non-infectious uveitis.Clinical & translational immunology · 2023Review
- Review
- Effects of Melatonin on Diabetic Neuropathy and Retinopathy.International journal of molecular sciences · 2021Review
- The Influence of Melatonin and Light on VEGF Secretion in Primary RPE Cells.Biomolecules · 2021Article
- Pro-inflammatory cytokine profile is present in the serum of Mexican patients with different stages of diabetic retinopathy secondary to type 2 diabetes.BMJ open ophthalmology · 2021Article
- A Topical Formulation of Melatoninergic Compounds Exerts Strong Hypotensive and Neuroprotective Effects in a Rat Model of Hypertensive Glaucoma.International journal of molecular sciences · 2020Article
- Mechanistic dissection of diabetic retinopathy using the protein-metabolite interactome.Journal of diabetes and metabolic disorders · 2020Article
- Astragalus polysaccharides suppresses high glucose-induced metabolic memory in retinal pigment epithelial cells through inhibiting mitochondrial dysfunction-induced apoptosis by regulating miR-195.Molecular medicine (Cambridge, Mass.) · 2019Article
- Comprehensive analysis of vitreous chemokines involved in ischemic retinal vein occlusion.Molecular vision · 2019Article
- Function of NLRP3 in the Pathogenesis and Development of Diabetic Nephropathy.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- The role of melatonin in the onset and progression of type 3 diabetes.Molecular brain · 2017Review
- Salvianolic acid B prevents steroid-induced osteonecrosis of the femoral head via PPARγ expression in rats.Experimental and therapeutic medicine · 2017Article
- Association between Aqueous Cytokines and Diabetic Retinopathy Stage.Journal of ophthalmology · 2017Article
- Entrance of the Tat protein of HIV-1 into human uterine cervical carcinoma cells causes upregulation of HPV-E6 expression and a decrease in p53 protein levels.Oncology letters · 2016Article
- High glucose promotes the migration of retinal pigment epithelial cells through increased oxidative stress and PEDF expression.American journal of physiology. Cell physiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeWe aimed to evaluate the effects of two immune regulatory factors, interleukin-4 (IL-4) and melatonin, on several inflammatory mediators that are involved in inflammation and angiogenesis in diabetic retinopathy (DR), in high glucose or interleukin-1β (IL-1β) induced primary human retinal endothelial cells (RECs) and human retinal pigment epithelial (RPE) cells.
methodsHuman RECs and RPE cells were cultured in 30 mM D-glucose or 10 ng/ml IL-1β, with or without the presence of 40 ng/ml IL-4 or 100 μM melatonin. The mRNA and protein levels of vascular endothelial growth factor (VEGF), intercellular cell adhesion molecule-1 (ICAM-1), matrix metalloproteinases 2 (MMP2), and matrix metalloproteinases 9 (MMP9) were measured using real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively.
resultsHigh glucose and IL-1β induced the expression of VEGF, ICAM-1, MMP2, and MMP9 in human RECs and RPE cells. IL-4 and melatonin downregulated the expression of VEGF, ICAM-1, MMP2, and MMP9 induced by high glucose and IL-1β.
conclusionsOur results demonstrated that IL-4 and melatonin inhibited inflammation and angiogenesis triggered by high glucose and IL-1β, which suggests that these immune regulatory factors may be of potential therapeutic value in DR.
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Identifiers
24991184PMC4077596W33260016What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.