Evidence map›Paper›PMID 24990896›Full record

ArticleAmerican journal of physiology. Renal physiology2014

Regulation of (pro)renin receptor expression in mIMCD via the GSK-3β-NFAT5-SIRT-1 signaling pathway.

Syed Quadri, Helmy M Siragy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Review
  3. The (pro)renin receptor in health and disease.Nature reviews. Nephrology · 2019
    Review
  4. Article
  5. Prorenin receptor mediates inflammation in renal ischemia.Clinical and experimental pharmacology & physiology · 2018
    Article
  6. The prorenin receptor in the cardiovascular system and beyond.American journal of physiology. Heart and circulatory physiology · 2018
    Review
  7. Article
  8. Article
  9. How Is the Brain Renin-Angiotensin System Regulated?Hypertension (Dallas, Tex. : 1979) · 2017
    Review
  10. Article
  11. Article
  12. Interaction of the renin angiotensin and cox systems in the kidney.Frontiers in bioscience (Scholar edition) · 2016
    Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Syed QuadriDivision of Endocrinology and Metabolism, University of Virginia Health System, Charlottesville, Virginia.
Helmy M SiragyDivision of Endocrinology and Metabolism, University of Virginia Health System, Charlottesville, Virginia hms7a@virginia.edu.
University of Virginia Health System · US

Funding

Prorenin Receptors Mediate Hypertension and Kidney Disease in DiabetesR01HL091535 · NHLBI · UNIVERSITY OF VIRGINIA · PI SIRAGY, HELMY M · 2008 to 2020
$5.1M
Renin receptor provoked renal inflammationR01DK078757 · NIDDK · UNIVERSITY OF VIRGINIA · PI SIRAGY, HELMY M · 2007 to 2016
$3.3M
NHLBI NIH HHS R01 HL091535NIDDK NIH HHS R01 DK078757
6 · The paper itself

Abstract

The localization and regulation of (pro)renin receptor (PRR) expression in kidney collecting duct cells are not well established. We hypothesized that low salt (LS) contributes to the regulation of PRR expression in these cells via the GSK-3β-NFAT5-sirtuin1 (SIRT-1) signaling pathway. Mouse inner medullary collecting duct (mIMCD) cells were treated with NaCl at 130 (normal salt; NS), 63 (LS), or 209 mM (high salt; HS) alone or in combination with NFAT5 scrambled small interfering (si) RNA, NFAT5 siRNA, or the SIRT-1 inhibitor EX-527. Compared with NS, LS increased the mRNA and protein expression of PRR by 71% and 69% (P < 0.05), and reduced phosphorylation of GSK-3β by 62% (P < 0.01), mRNA and protein expressions of NFAT5 by 65% and 45% (P < 0.05), and SIRT-1 by 44% and 50% (P < 0.01), respectively. LS also enhanced p65 NF-κB by 102% (P < 0.01). Treatment with HS significantly reduced the mRNA and protein expression of PRR by 32% and 23% (P < 0.05), and increased the mRNA and protein expression of NFAT5 by 39% and 45% (P < 0.05) and SIRT-1 by 51% and 56% (P < 0.05), respectively. HS+NFAT5 siRNA reduced the mRNA and protein expression of NFAT5 by 51% and 35% (P < 0.01) and increased the mRNA and protein expression of PRR by 148% and 70% (P < 0.01), respectively. HS+EX-527 significantly increased the mRNA and protein expression of PRR by 96% and 58% (P < 0.05), respectively. We conclude that expression of PRR in mIMCD cells is regulated by the GSK-3β-NFAT5- SIRT-1 signaling pathway.

Indexed as

AnimalsCarbazolesCell LineDose-Response Relationship, DrugGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaIn Vitro TechniquesKidney Tubules, CollectingMicePhosphorylationProrenin ReceptorReceptors, Cell SurfaceRNA, Small InterferingSignal TransductionSirtuin 1Sodium Chloride6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamideCarbazolesGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaGsk3b protein, mouseNfat5 protein, mouseProrenin ReceptorReceptors, Cell SurfaceRela protein, mouseRNA, Small InterferingSirt1 protein, mouseSirtuin 1Sodium ChlorideTranscription Factor RelATranscription Factorsinner medullary collecting ductNFAT5(pro)renin receptorSIRT-1

Identifiers

PMID24990896
PMCPMC4841440
OpenAlexW2047605577

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.