Evidence map›Paper›PMID 24989612›Full record

ArticleJournal of clinical microbiology2014

Same-day subtyping of Campylobacter jejuni and C. coli isolates by use of multiplex ligation-dependent probe amplification-binary typing.

Angela J Cornelius, Olivier Vandenberg, Beth Robson, Brent J Gilpin, Stephanie M Brandt, Paula Scholes, Delphine Martiny, Philip E Carter, Paul van Vught, Jan Schouten and 1 more

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Angela J CorneliusInstitute of Environmental Science and Research (ESR), Christchurch, New Zealand angela.cornelius@esr.cri.nz.
Olivier VandenbergNational Reference Center for Campylobacter, Saint Pierre University Hospital, Brussels, Belgium Ecole de Santé Publique, Université Libre de Bruxelles, Brussels, Belgium.
Beth RobsonInstitute of Environmental Science and Research (ESR), Christchurch, New Zealand.
Brent J GilpinInstitute of Environmental Science and Research (ESR), Christchurch, New Zealand.
Stephanie M BrandtInstitute of Environmental Science and Research (ESR), Christchurch, New Zealand.
Paula ScholesInstitute of Environmental Science and Research (ESR), Christchurch, New Zealand.
Delphine MartinyNational Reference Center for Campylobacter, Saint Pierre University Hospital, Brussels, Belgium.
Philip E CarterInstitute of Environmental Science and Research (ESR), Wellington, New Zealand.
Paul van VughtMRC-Holland, Amsterdam, The Netherlands.
Jan SchoutenMRC-Holland, Amsterdam, The Netherlands.
Stephen L W OnInstitute of Environmental Science and Research (ESR), Christchurch, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Campylobacteriosis is the most commonly reported form of human bacterial gastroenteritis in the world. Sound identification of infectious sources requires subtyping, but the most widely used methods have turnaround times measured in days and require specialist equipment and skills. A multiplex ligation-dependent probe amplification-binary typing (MBiT) assay was developed for subtyping Campylobacter jejuni and Campylobacter coli. It was tested on 245 isolates, including recent isolates from Belgium and New Zealand, and compared to multilocus sequence typing (MLST). When used in an outbreak setting, MBiT identified the predominant genotype and possible additional cases days before pulsed-field gel electrophoresis (PFGE) results were available. MBiT was more discriminatory than MLST and, being a single assay with results produced within 6 h, was more rapid and cost-effective than both MLST and PFGE. In addition, MBiT requires only basic molecular biology equipment and skills.

Indexed as

BelgiumCampylobacter coliCampylobacter InfectionsCampylobacter jejuniElectrophoresis, Gel, Pulsed-FieldHumansMolecular TypingMultiplex Polymerase Chain ReactionNew ZealandSensitivity and SpecificityTime Factors

Identifiers

PMID24989612
PMCPMC4313161

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.