Evidence map›Paper›PMID 24988349›Full record

ArticleBiophysical journal2014

Tensional homeostasis in single fibroblasts.

Kevin D Webster, Win Pin Ng, Daniel A Fletcher

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 125 citations in OpenAlex.

  1. Article
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  4. Review
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  6. Cell-extracellular matrix mechanotransduction in 3D.Nature reviews. Molecular cell biology · 2023
    Review
  7. Article
  8. Article
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  11. Article
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  18. Article
  19. The mechanics of fibrillar collagen extracellular matrix.Cell reports. Physical science · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Kevin D WebsterBiophysics Graduate Group, University of California, Berkeley, California; Department of Bioengineering, University of California, Berkeley, California.
Win Pin NgDepartment of Bioengineering, University of California, Berkeley, California; University of California Berkeley/University of California San Francisco Graduate Group in Bioengineering, Berkeley, California.
Daniel A FletcherBiophysics Graduate Group, University of California, Berkeley, California; Department of Bioengineering, University of California, Berkeley, California; University of California Berkeley/University of California San Francisco Graduate Group in Bioengineering, Berkeley, California; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California. Electronic address: fletch@berkeley.edu.
Lawrence Berkeley National Laboratory · USUniversity of California, Berkeley · USUniversity of California, San Francisco · US

Funding

Mechanical Regulation of Actin NetworksR01GM074751 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI FLETCHER, DANIEL A · 2008 to 2015
$2.4M
NIGMS NIH HHS R01 GM074751
6 · The paper itself

Abstract

Adherent cells generate forces through acto-myosin contraction to move, change shape, and sense the mechanical properties of their environment. They are thought to maintain defined levels of tension with their surroundings despite mechanical perturbations that could change tension, a concept known as tensional homeostasis. Misregulation of tensional homeostasis has been proposed to drive disorganization of tissues and promote progression of diseases such as cancer. However, whether tensional homeostasis operates at the single cell level is unclear. Here, we directly test the ability of single fibroblast cells to regulate tension when subjected to mechanical displacements in the absence of changes to spread area or substrate elasticity. We use a feedback-controlled atomic force microscope to measure and modulate forces and displacements of individual contracting cells as they spread on a fibronectin-patterned atomic-force microscope cantilever and coverslip. We find that the cells reach a steady-state contraction force and height that is insensitive to stiffness changes as they fill the micropatterned areas. Rather than maintaining a constant tension, the fibroblasts altered their contraction force in response to mechanical displacement in a strain-rate-dependent manner, leading to a new and stable steady-state force and height. This response is influenced by overexpression of the actin crosslinker α-actinin, and rheology measurements reveal that changes in cell elasticity are also strain- rate-dependent. Our finding of tensional buffering, rather than homeostasis, allows cells to transition between different tensional states depending on how they are displaced, permitting distinct responses to slow deformations during tissue growth and rapid deformations associated with injury.

Indexed as

HomeostasisTensile StrengthActininAnimalsElasticityFibroblastsMiceNIH 3T3 CellsActinin

Identifiers

PMID24988349
PMCPMC4119263
OpenAlexW1974954154

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.