ArticleBiophysical journal2014
Quantifying protein-protein interactions of peripheral membrane proteins by fluorescence brightness analysis.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 25 citations in OpenAlex.
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- Benchmarking of novel green fluorescent proteins for the quantification of protein oligomerization in living cells.PloS one · 2023Article
- Partitioning of ribonucleoprotein complexes from the cellular actin cortex.Science advances · 2022Article
- Raf promotes dimerization of the Ras G-domain with increased allosteric connections.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Differentiating Luminal and Membrane-Associated Nuclear Envelope Proteins.Biophysical journal · 2020Article
- Sensitive Detection of Protein Binding to the Plasma Membrane with Dual-Color Z-Scan Fluorescence.Biophysical journal · 2020Article
- Identifying Heteroprotein Complexes in the Nuclear Envelope.Biophysical journal · 2020Article
- Quantitative modeling of self-oligomerization of proteins in the nuclear envelope by fluorescence fluctuation analysis.Analytical biochemistry · 2019Article
- Protein oligomerization and mobility within the nuclear envelope evaluated by the time-shifted mean-segmented Q factor.Methods (San Diego, Calif.) · 2019Article
- Fluorescence fluctuation spectroscopy reveals differential SUN protein oligomerization in living cells.Molecular biology of the cell · 2018Article
- Eph-ephrin signaling modulated by polymerization and condensation of receptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Quantitative Brightness Analysis of Protein Oligomerization in the Nuclear Envelope.Biophysical journal · 2017Article
- Covalent Ras Dimerization on Membrane Surfaces through Photosensitized Oxidation.Journal of the American Chemical Society · 2016Article
- Z-scan fluorescence profile deconvolution of cytosolic and membrane-associated protein populations.Analytical biochemistry · 2015Article
- In situ quantification of protein binding to the plasma membrane.Biophysical journal · 2015Article
- Article
- Scan and conquer: a novel approach to the analysis of interactions by molecular brightness determination.Biophysical journal · 2014Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescently labeled proteins that are found both in the cytoplasm and at the plasma membrane, such as peripheral membrane proteins, create stratified fluorescent layers that present a challenging environment for brightness studies with fluorescence fluctuation spectroscopy. The geometry of each layer along with fluorescence and brightness contributions from adjacent layers generates a convoluted raw brightness that conceals the underlying brightness of each individual layer. Because the brightness at a layer establishes the oligomeric state of the fluorescently labeled protein at said layer, we developed a method that connects the experimental raw brightness with the physical brightness at each layered compartment. The technique determines the oligomerization in each compartment from an axial intensity scan through the sample, followed by a fluorescence fluctuation spectroscopy measurement at each layer. We experimentally verify the technique with H-Ras-EGFP as a model system and determine its oligomeric state at both the plasma membrane and in the cytoplasm. Furthermore, we study the oligomerization of the Gag matrix domain of Human T-lymphotropic virus Type 1. The matrix domain targets the Gag polyprotein to the plasma membrane where, subsequently, viral assembly occurs. We determine the oligomerization of matrix in the cytoplasm and observe the onset of protein-protein interactions at the membrane. These observations shed light on the early assembly steps of the retrovirus.
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