ArticleTheScientificWorldJournal2014
Culture and characterization of microglia from the adult murine retina.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- ATP-P2XJournal of neuroinflammation · 2023Article
- Neuroinflammation, Microglia and Implications for Retinal Ganglion Cell Survival and Axon Regeneration in Traumatic Optic Neuropathy.Frontiers in immunology · 2022Review
- Effects of a New Combination of Natural Extracts on Glaucoma-Related Retinal Degeneration.Foods (Basel, Switzerland) · 2021Article
- Spleen Tyrosine Kinase Mediates Microglial Activation in Mice With Diabetic Retinopathy.Translational vision science & technology · 2021Article
- Dynamics of Cyclooxygenase-1 Positive Microglia/Macrophage in the Retina of Pathological Model Mice as a Biomarker of the Retinal Inflammatory Diseases.International journal of molecular sciences · 2021Article
- Heme Causes Pain in Sickle MiceAntioxidants & redox signaling · 2021Article
- Clock Gene Nr1d1 Alleviates Retinal Inflammation Through Repression of Hmga2 in Microglia.Journal of inflammation research · 2021Article
- Isolation and Characterization of Primary Retinal Microglia From the Human Post-mortem Eyes for Future Studies of Ocular Diseases.Frontiers in cellular neuroscience · 2021Article
- Phenotypic Differences in Primary Murine Microglia Treated with NOD1, NOD2, and NOD1/2 Agonists.Journal of molecular neuroscience : MN · 2020Article
- Characterization of a functionally active primary microglial cell culture from the pig retina.Experimental eye research · 2019Article
- Glucose transporter 1 critically controls microglial activation through facilitating glycolysis.Molecular neurodegeneration · 2019Article
- Loss of IL-10 Promotes Differentiation of Microglia to a M1 Phenotype.Frontiers in cellular neuroscience · 2019Article
- Inhibition of Notch1 Signaling Alleviates Endotoxin-Induced Inflammation Through Modulating Retinal Microglia Polarization.Frontiers in immunology · 2019Article
- TGF-β1 modulates microglial phenotype and promotes recovery after intracerebral hemorrhage.The Journal of clinical investigation · 2017Article
- Hyalocytes in idiopathic epiretinal membranes: a correlative light and electron microscopic study.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo develop a protocol for isolating and culturing murine adult retinal microglia and to characterize the phenotype and function of the cultured cells.
methodRetinal single-cell suspensions were prepared from adult MF1 mice. Culture conditions including culture medium, growth factors, seeding cell density, and purification of microglia from the mixed cultures were optimised. Cultured retinal microglial cells were phenotyped using the surface markers CD45, CD11b, and F4/80. Their ability to secrete proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation was examined using cytometric bead array (CBA) assay.
resultsHigher yield was obtained when retinal single-cell suspension was cultured at the density of 0.75 × 10(6) cells per cm(2) in Dulbecco's modified Eagle medium (DMEM)/F12 + Glutamax supplement with 20% fetal calf serum (FCS) and 20% L929 supernatant. We identified day 10 to be the optimum day of microglial isolation. Over 98% of the cells isolated were positive for CD45, CD11b, and F4/80. After stimulating with LPS they were able to secrete proinflammatory cytokines such as IL-6 and TNF- α and express CD86, CD40, and MHC-II.
conclusionWe have developed a simple method for isolating and culturing retinal microglia from adult mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.