ArticleBioMed research international2014
Gold nanoparticles inhibit VEGF165-induced migration and tube formation of endothelial cells via the Akt pathway.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.
- Antiangiogenic properties of nanoparticles: a systematic review.International journal of nanomedicine · 2019Pooled it
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- MiRNA-based drugs: challenges and delivery strategies.Applied microbiology and biotechnology · 2025Review
- Gold nanoparticles in cardiovascular medicine: current applications and future directions in nano-cardiology.Annals of medicine and surgery (2012) · 2025Review
- Development of a Decellularized Urinary Bladder Matrix and Heparin-Based Cryogel for Promoting Angiogenesis.Macromolecular bioscience · 2025Article
- Surface functionalization of microscaffolds produced by high-resolution 3D printing: A new layer of freedom.Materials today. Bio · 2025Article
- Intraocular drug delivery systems for Diabetic retinopathy: Current and future prospective.BioImpacts : BI · 2025Review
- Anticancer Action of Silver Nanoparticles in SKBR3 Breast Cancer Cells through Promotion of Oxidative Stress and Apoptosis.BioMed research international · 2024Article
- Green biologically synthesized metal nanoparticles: biological applications, optimizations and future prospects.Future science OA · 2024Review
- Methods of miRNA delivery and possibilities of their application in neuro-oncology.Non-coding RNA research · 2023Review
- The Role of Silver Nanoparticles in the Diagnosis and Treatment of Cancer: Are There Any Perspectives for the Future?Life (Basel, Switzerland) · 2023Review
- A More Biomimetic Cell Migration Assay with High Reliability and Its Applications.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Construction of nanofibrous scaffolds with interconnected perfusable microchannel networks for engineering of vascularized bone tissue.Bioactive materials · 2021Article
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- Cancer-Nano-Interaction: From Cellular Uptake to Mechanobiological Responses.International journal of molecular sciences · 2021Review
- Gold Nanoparticles Affect Pericyte Biology and Capillary Tube Formation.Pharmaceutics · 2021Article
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- The Inhibitory Effects of Gold Nanoparticles on VEGF-A-Induced Cell Migration in Choroid-Retina Endothelial Cells.International journal of molecular sciences · 2019Article
- Exploiting Current Understanding of Hypoxia Mediated Tumour Progression for Nanotherapeutic Development.Cancers · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The early stages of angiogenesis can be divided into three steps: endothelial cell proliferation, migration, and tube formation. Vascular endothelial growth factor (VEGF) is considered the most important proangiogenic factor; in particular, VEGF165 plays a critical role in angiogenesis. Here, we evaluated whether gold nanoparticles (AuNPs) could inhibit the VEGF165-induced human umbilical vein endothelial cell (HUVEC) migration and tube formation. AuNPs and VEGF165 were coincubated overnight at 4°C, after which the effects on cell migration and tube formation were assessed. Cell migration was assessed using a modified wound-healing assay and a transwell chamber assay; tube formation was assessed using a capillary-like tube formation assay and a chick chorioallantoic membrane (CAM) assay. We additionally detected the cell surface morphology and ultrastructure using atomic force microscopy (AFM). Furthermore, Akt phosphorylation downstream of VEGFR-2/PI3K in HUVECs was determined in a Western blot analysis. Our study demonstrated that AuNPs significantly inhibited VEGF165-induced HUVEC migration and tube formation by affecting the cell surface ultrastructure, cytoskeleton and might have inhibited angiogenesis via the Akt pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.